Among the rust diseases, leaf rust of wheat caused by Puccinia triticina, is the most prevalent worldwide and causes significant yield losses. This study aimed to determine the genomic location of loci that control adult plant resistance (APR) to leaf rust in the pre-Green Revolution landrace accession, Aus27506, from the “Watkins Collection”. An Aus27506/Aus27229-derived F₇ recombinant inbred line (RIL) population was screened under field conditions across three cropping seasons and genotyped with the iSelect 90K Infinium SNP bead chip array. One quantitative trait loci (QTL) on each of the chromosomes 1BL, 2B and 2DL explained most of the leaf rust response variation in the RIL population, and these were named QLr.sun-1BL, QLr.sun-2B and QLr.sun-2DL, respectively. QLr.sun-1BL and QLr.sun-2DL were contributed by Aus27506. QLr.sun-1BL is likely Lr46, while QLr.sun-2DL appeared to be a new APR locus. The alternate parent, Aus27229, carried the putatively new APR locus QLr.sun-2B. The comparison of average severities among RILs carrying these QTL in different combinations indicated that QLr.sun-2B does not interact with either of the other two QTL; however, the combination of QLr.sun-1BL and QLr.sun-2DL reduced disease severity significantly. In planta fungal quantification assays validated these results. The RILs carrying QLr.sun-1BL and QLr.sun-2DL did not differ significantly from the parent Aus27506 in terms of resistance. Aus27506 can be used as a source of adult plant leaf rust resistance in breeding programs.

中文翻译：

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Aus27506中成年植物叶片抗锈性的定位及植物内真菌生物量积累的潜在位点验证

间的锈病，引起小麦的叶锈病小麦叶锈菌，是最常见的全球并导致显著产量损失。这项研究旨在确定控制基因的基因组位置，以控制“绿色革命”前地方品种（Aus27506）“ Watkins Collection”中的成年植物对叶锈病的抗性。在三个种植季节的田间条件下筛选了Aus27506 / Aus27229衍生的F ₇重组自交系（RIL）群体，并使用iSelect 90K Infinium SNP珠芯片阵列进行了基因分型。染色体1BL，2B和2DL上每个染色体上的一个数量性状基因座（QTL）解释了RIL群体中大部分的叶锈病响应变异，它们被称为QLr.sun-1BL，QLr.sun-2B和QLr.sun-2DL。QLr.sun-1BL和QLr.sun-2DL由Aus27506贡献。QLr.sun-1BL可能是Lr46，而QLr.sun-2DL似乎是一个新的APR位点。替代亲本Aus27229携带了新的APR基因座QLr.sun-2B。比较以不同组合携带这些QTL的RIL之间的平均严重性，这表明QLr.sun-2B不会与其他两个QTL中的任何一个相互作用。但是，QLr.sun-1BL和QLr.sun-2DL的组合大大降低了疾病严重程度。在植物中的真菌定量测定验证了这些结果。携带QLr.sun-1BL和QLr.sun-2DL的RIL在抗性方面与亲本Aus27506没有显着差异。在育种计划中，Aus27506可用作成年植物叶片防锈的来源。