An investigation of the ecological niches and seasonal nature of Clostridium estertheticum and Clostridium gasigenes in the Irish beef farm environment,Letters in Applied Microbiology

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An investigation of the ecological niches and seasonal nature of Clostridium estertheticum and Clostridium gasigenes in the Irish beef farm environment
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-10-01 , DOI: 10.1111/lam.13344
E Esteves _{1,

2,

3} , P Whyte ₂ , T B Gupta ₃ , D J Bolton ₁

Affiliation

Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. On each of five different beef farms, faecal (10), soil (5), silage (5), air (5), bedding straw (5), drinking water (5) and puddle/ditch water (5) samples were collected during Spring, Summer, Autumn and Winter and tested for C. estertheticum and C. gasigenes using culture (direct plating and enrichment) and molecular (conventional PCR and quantitative PCR (qPCR)) based techniques. C. estertheticum and C. gasigenes were detected in all sample types, with qPCR detection rates ranging from 4% to 50% and at concentrations of up to 1·5 log10 CFU per g and 3·5 log10 CFU per g, respectively. The impact of season was not clear as the results were mixed depending on the detection method used. It was concluded that BPS‐causing C. estertheticum and C. gasigenes are widely distributed in the beef farm environment.

中文翻译：

爱尔兰养牛场环境中酯化梭菌和气化梭菌的生态位和季节性特征的调查

真空包装牛肉的吹塑变质 (BPS) 是由耐冷和嗜冷梭菌属物种引起的，主要是酯化梭菌和 Clostridium gasigenes。本研究的目的是调查环境生态位和季节对这些 BPS 梭菌的影响。在爱尔兰牛肉农场。在五个不同的养牛场中，分别收集了粪便 (10)、土壤 (5)、青贮饲料 (5)、空气 (5)、垫草 (5)、饮用水 (5) 和水坑/沟渠水 (5) 样品在春季、夏季、秋季和冬季，并使用基于培养（直接接种和富集）和分子（常规 PCR 和定量 PCR (qPCR)）的技术测试 C.estertheticum 和 C.gasigenes。在所有样品类型中均检测到 C.estertheticum 和 C.gasigenes，qPCR 检测率从 4% 到 50% 不等，浓度分别高达 1·5 log10 CFU/g 和 3·5 log10 CFU/g。季节的影响并不清楚，因为根据所使用的检测方法，结果是混合的。得出的结论是，引起 BPS 的 C.estertheticum 和 C.gasigenes 广泛分布在养牛场环境中。

更新日期：2020-10-01

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