Trichomes are universal specific structures originating from nearly all terrestrial plants. Although quantities of long non‐coding RNAs (lncRNAs) have been identified in many plant species, the role of lncRNAs in trichome formation still remains unknown. Here, we identified a total of 1303 lncRNAs in the young stems of woolly mutant LA3560 (Wo) and its non‐woolly segregants (WT). Out of these lncRNAs, 86 lncRNAs were obviously upregulated in Wo and 110 lncRNAs were downregulated. We determined that seven lncRNAs were highly expressed in stem trichomes compared to trichome‐free stems and several other tissues of LA3560 by a quantitative reverse transcriptase‐polymerase chain reaction, including lncRNA000746, lncRNA000170, lncRNA000277, lncRNA000774, lncRNA000756, lncRNA000100, and lncRNA000898. Transgenic experiments revealed that overexpression of lncRNA000170 inhibited type I trichome formation on the lower stems of the adult transgenic plants. We further determined that lncRNA000170 was transcribed from the complementary strand of Solyc10g006360, for which expression can be induced by lncRNA000170 in its overexpression lines and woolly mutants. Solyc10g006360 overexpression also caused type I trichome decrease. In addition, several trichome regulators, such as Wo, H, SlCycB2, and SlCycB3, were markedly downregulated in lncRNA000170 overexpression lines. These findings demonstrate that lncRNA000170 may be involved in the regulatory pathway mediated by these trichome regulators.

中文翻译：

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转录组学和功能分析揭示了lncRNA000170在番茄多细胞毛状体形成中的调控作用。

毛滴虫是起源于几乎所有陆生植物的通用特定结构。尽管在许多植物物种中已经鉴定出大量的长非编码RNA（lncRNA），但是lncRNA在毛状体形成中的作用仍然未知。在这里，我们在羊毛突变体LA3560（Wo）及其非羊毛分离物（WT）的年轻茎中鉴定出总共1303个lncRNA。在这些lncRNA中，有86个lncRNA在Wo中明显上调。并下调了110个lncRNA。通过定量逆转录聚合酶链反应，我们确定与无毛状体茎和LA3560的其他多个组织相比，干毛状体中有7种lncRNA高表达，包括lncRNA000746，lncRNA000170，lncRNA000277，lncRNA000774，lncRNA000756，lncRNA000100和lncRNA000898。转基因实验表明，lncRNA000170的过表达抑制了成年转基因植物下部茎上的I型毛状体形成。我们进一步确定lncRNA000170是从Solyc10g006360的互补链转录而来的，lncRNA000170可以在其过度表达系和woolly突变体中诱导表达。Solyc10g006360过表达也导致I型毛状体减少。此外，一些三脚架稳压器，例如Wo，H，SlCycB2和SlCycB3在lncRNA000170过表达株系中显着下调。这些发现表明lncRNA000170可能参与了这些毛状体调节剂介导的调节途径。