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A WRKY transcription factor, TaWRKY40-D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat.
Plant Biology ( IF 4.2 ) Pub Date : 2020-07-01 , DOI: 10.1111/plb.13155 L Zhao 1 , W Zhang 1 , Q Song 2 , Y Xuan 1 , K Li 1 , L Cheng 1 , H Qiao 1 , G Wang 1 , C Zhou 1
中文翻译：
WRKY转录因子TaWRKY40-D促进小麦中与茉莉酸和脱落酸途径相关的叶片衰老。
更新日期：2020-07-01
Plant Biology ( IF 4.2 ) Pub Date : 2020-07-01 , DOI: 10.1111/plb.13155 L Zhao 1 , W Zhang 1 , Q Song 2 , Y Xuan 1 , K Li 1 , L Cheng 1 , H Qiao 1 , G Wang 1 , C Zhou 1
Affiliation
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- Leaf senescence is a complex and precise regulatory process that is correlated with numerous internal and environmental factors. Leaf senescence is tightly related to the redistribution of nutrients, which significantly affects productivity and quality, especially in crops. Evidence shows that the mediation of transcriptional regulation by WRKY transcription factors is vital for the fine‐tuning of leaf senescence. However, the underlying mechanisms of the involvement of WRKY in leaf senescence are still unclear in wheat.
- Using RNA sequencing data, we isolated a novel WRKY transcription factor, TaWRKY40‐D, which localizes in the nucleus and is basically induced by the progression of leaf senescence.
- TaWRKY40‐D is a promoter of natural and dark‐induced leaf senescence in transgenic Arabidopsis thaliana and wheat. We also demonstrated a positive response of TaWRKY40‐D in wheat upon jasmonic acid (JA) and abscisic acid (ABA) treatment. Consistent with this, the detached leaves of TaWRKY40‐D VIGS (virus‐induced gene silencing) wheat plants showed a stay‐green phenotype, while TaWRKY40‐D overexpressing Arabidopsis plants showed premature leaf senescence after JA and ABA treatment. Moreover, our results revealed that TaWRKY40‐D positively regulates leaf senescence, possibly by altering the biosynthesis and signalling of JA and ABA pathway genes.
- Together, our results suggest a new regulator of JA‐ and ABA‐related leaf senescence, as well as a new candidate gene that can be used for molecular breeding in wheat.
中文翻译：
WRKY转录因子TaWRKY40-D促进小麦中与茉莉酸和脱落酸途径相关的叶片衰老。
- 叶片衰老是一个复杂而精确的调节过程，与许多内部和环境因素相关。叶片衰老与养分的重新分配紧密相关，这会严重影响生产力和质量，尤其是在作物中。有证据表明，由WRKY转录因子介导的转录调控对于微调叶片衰老至关重要。然而，小麦中WRKY参与叶片衰老的潜在机制仍不清楚。
- 使用RNA测序数据，我们分离了一种新型的WRKY转录因子TaWRKY40-D，它位于细胞核中，并且基本上是由叶片衰老的过程诱导的。
- TaWRKY40‐D是转基因拟南芥和小麦中自然和黑暗诱导的叶片衰老的启动子。我们还证明了茉莉酸（JA）和脱落酸（ABA）处理后TaWRKY40‐D在小麦中呈阳性反应。与此相一致的是，TaWRKY40‐D VIGS（病毒诱导的基因沉默）小麦植物的离体叶片表现出保持绿色的表型，而过表达TaWRKY40‐D的拟南芥植物在JA和ABA处理后表现出过早的叶片衰老。此外，我们的结果表明TaWRKY40-D可能通过改变JA和ABA途径基因的生物合成和信号传导来正向调节叶片的衰老。
- 总之，我们的结果表明，JA和ABA相关叶片衰老的新调节剂，以及可用于小麦分子育种的新候选基因。
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