Polycaprolactone (PCL) fiber mats with different surface modifications were functionalized with a chitosan nanogel coating to attach the growth factor human bone morphogenetic protein 2 (BMP‐2). Three different hydrophilic surface modifications were compared with regard to the binding and in vitro release of BMP‐2. The type of surface modification and the specific surface area derived from the fiber thickness had an important influence on the degree of protein loading. Coating the PCL fibers with polydopamine resulted in the binding of the largest BMP‐2 quantity per surface area. However, most of the binding was irreversible over the investigated period of time, causing a low release in vitro. PCL fiber mats with a chitosan‐graft‐PCL coating and an additional alginate layer, as well as PCL fiber mats with an air plasma surface modification boundless BMP‐2, but the immobilized protein could almost completely be released. With polydopamine and plasma modifications as well as with unmodified PCL, high amounts of BMP‐2 could also be attached directly to the surface. Integration of BMP‐2 into the chitosan nanogel functionalization considerably increased binding on all hydrophilized surfaces and resulted in a sustained release with an initial burst release of BMP‐2 without detectable loss of bioactivity in vitro.

中文翻译：

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通过不同的聚己内酯表面改性改变 BMP-2 从壳聚糖纳米凝胶功能化聚己内酯纤维垫中的缓释

具有不同表面改性的聚己内酯 (PCL) 纤维垫用壳聚糖纳米凝胶涂层功能化，以附着生长因子人骨形态发生蛋白 2 (BMP-2)。在 BMP-2的结合和体外释放方面比较了三种不同的亲水性表面修饰。表面改性的类型和由纤维厚度得出的比表面积对蛋白质负载程度有重要影响。用聚多巴胺涂覆 PCL 纤维导致每表面积结合最大量的 BMP-2。然而，大部分结合在研究期间是不可逆的，导致体外低释放. 具有壳聚糖-接枝-PCL 涂层和额外藻酸盐层的 PCL 纤维垫，以及具有空气等离子表面改性的无边界 BMP-2 的 PCL 纤维垫，但固定化的蛋白质几乎可以完全释放。通过聚多巴胺和血浆修饰以及未修饰的 PCL，大量的 BMP-2 也可以直接附着在表面上。将 BMP-2 整合到壳聚糖纳米凝胶功能化中显着增加了所有亲水化表面的结合，并导致 BMP-2 的初始突发释放持续释放，而体外没有可检测到的生物活性损失。