Current hyaluronic acid‐based hydrogels often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective biomedical and tissue engineering applications. Provision of the cell‐adhesive surface is an important requirement to improve its biocompatibility. An aqueous solution of hyaluronic acid possessing phenolic hydroxyl (HA‐Ph) moieties is gellable via a horseradish peroxidase (HRP)‐catalyzed oxidative cross‐linking reaction. This study evaluates the effect of different degrees of cross‐linked Ph moieties on cellular adhesiveness and proliferation on the resultant enzymatically cross‐linked HA‐Ph hydrogels. Mechanical characterization demonstrated that the compression force of engineered hydrogels could be tuned in the range of 0.05–35 N by changing conjugated Ph moieties in the precursor formulation. The water contact angle and water content show hydrophobicity of hydrogels increased with increasing content of cross‐linked Ph groups. The seeded mouse embryo fibroblast‐like cell line and human cervical cancer cell line, on the HA‐Ph hydrogel, proved cell attachment and spreading with a high content of cross‐linked Ph groups. The HA‐Ph with a higher degree of Ph moieties shows the maximum degree of cell adhesion, spreading, and proliferation which presents this hydrogel as a suitable biomaterial for biomedical and tissue engineering applications.

中文翻译：

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通过不同程度的苯酚部分交联控制透明质酸基水凝胶中的细胞粘附性

目前基于透明质酸的水凝胶通常会对封装的细胞产生细胞毒性，并且缺乏有效的生物医学和组织工程应用所需的粘附特性。提供细胞粘附表面是提高其生物相容性的重要要求。具有酚羟基 (HA-Ph) 部分的透明质酸水溶液可通过辣根过氧化物酶 (HRP) 催化的氧化交联反应凝胶化。本研究评估了不同程度的交联 Ph 部分对所得酶交联 HA-Ph 水凝胶的细胞粘附性和增殖的影响。机械表征表明，工程水凝胶的压缩力可以通过改变前体配方中的共轭 Ph 部分在 0.05-35 N 的范围内进行调整。水接触角和水含量表明水凝胶的疏水性随着交联的Ph基团含量的增加而增加。在 HA-Ph 水凝胶上接种的小鼠胚胎成纤维细胞样细胞系和人宫颈癌细胞系证明细胞附着和扩散具有高含量的交联 Ph 基团。具有更高程度的 Ph 部分的 HA-Ph 显示出最大程度的细胞粘附、扩散和增殖，这表明该水凝胶是适合生物医学和组织工程应用的生物材料。证明细胞附着和扩散具有高含量的交联Ph基团。具有更高程度的 Ph 部分的 HA-Ph 显示出最大程度的细胞粘附、扩散和增殖，这表明该水凝胶是适合生物医学和组织工程应用的生物材料。证明细胞附着和扩散具有高含量的交联Ph基团。具有更高程度的 Ph 部分的 HA-Ph 显示出最大程度的细胞粘附、扩散和增殖，这表明该水凝胶是适合生物医学和组织工程应用的生物材料。