Acid bone lysates (ABLs) represent the growth factors and other molecules released during autologous graft resorption. However, the impact of these bone‐derived growth factors on the healing of bone defects has not yet been investigated. The aim of the present study was, therefore, to examine the impact of ABLs adsorbed to collagen membranes on bone regeneration. To this end, in 16 female Sprague Dawley rats, a standardized 5‐mm‐diameter critical size defect on the calvarial bone was created. The defects were covered with collagen membranes that had been soaked either in serum‐free media or ABLs followed by lyophilization. After a healing period of 4 weeks, micro‐computed tomography (μCT) and histological analyses by means of undecalcified thin ground sections were performed. μCT analysis of the inner 4 mm of the calvaria defect showed a greater bone defect coverage in the control group when compared to ABL group, 29.8% (confidence interval [CI]: 17.7–50.3) versus 5.6% (CI: 1.0–29.8, p = .03), respectively. Moreover, we found significantly more absolute bone volume (BV) in the control group when compared to ABL group, 0.59 mm³ (CI: 0.27–1.25) versus 0.07 mm³ (CI: 0.06–0.59, p = .04), respectively. Histomorphometry confirmed these findings with a relative BV in the central compartment of 14.1% (CI: 8.4–20.6) versus 5.6% (CI: 3.4–7.9, p = .004), respectively. These findings indicate that bone‐derived growth factors contained in ABLs are able to attenuate bone regeneration within collagen membranes.

中文翻译：

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酸性骨裂解物减少大鼠颅盖骨缺损的骨再生

酸性骨溶解物 (ABL) 代表自体移植物吸收过程中释放的生长因子和其他分子。然而，尚未研究这些骨源性生长因子对骨缺损愈合的影响。因此，本研究的目的是检查吸附在胶原膜上的 ABL 对骨再生的影响。为此，在 16 只雌性 Sprague Dawley 大鼠中，在颅骨上创建了一个标准化的 5 mm 直径临界尺寸缺陷。缺陷被胶原膜覆盖，胶原膜已浸泡在无血清培养基或 ABL 中，然后冻干。在 4 周的愈合期后，进行了微计算机断层扫描 (μCT) 和未脱钙薄地面切片的组织学分析。p = .03)，分别。此外，我们发现与 ABL 组相比，对照组的绝对骨体积 (BV) 显着增加，分别为 0.59 mm ³ (CI: 0.27–1.25) 和 0.07 mm ³ (CI: 0.06–0.59, p = .04) . 组织形态学证实了这些发现，中央隔室的相对 BV 分别为 14.1% (CI: 8.4–20.6) 和 5.6% (CI: 3.4–7.9, p = .004)。这些发现表明，ABL 中含有的骨衍生生长因子能够减弱胶原膜内的骨再生。