In our previous study, we found that pumilacidin‐like cyclic lipopeptides (CLPs) derived from marine bacterium Bacillus sp. strain 176 significantly suppressed the mobile capability and virulence of Vibrio alginolyticus. Here, to further disclose the mechanism of CLPs inhibiting the motility of V. alginolyticus, we first applied transcriptomic analysis to V. alginolyticus treated with or without CLPs. The transcriptomic results showed that the expression of several important components of the Na⁺‐driven flagellar motor closely related to bacterial motility were markedly suppressed, suggesting that the structure and function of Na⁺‐driven flagellar motor might be disabled by CLPs. The transcriptomic data were further analysed by the protein–protein interaction network, and the results supported that MotX, one of the essential components of Na⁺‐driven flagellar motor was most likely the action target of CLPs. In combination of gene knockout, electrophoretic mobility shift assay and immunoblotting techniques, CLPs were demonstrated to affect the rotation of flagella of Vibrio alginolyticus via direct interacting with the Na⁺‐driven flagellar motor component MotX, which eventually inhibited the bacterial motility. Interestingly, homologues of MotX were found broadly distributed and highly conserved in different pathogenic species, which extends the application range of CLPs as an antibacterial drug targeting bacterial motility in many pathogens.

中文翻译：

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环状脂肽通过靶向Na +驱动的鞭毛运动成分MotX来抑制溶藻弧菌的运动。

在我们以前的研究中，我们发现了源自海洋细菌芽孢杆菌属（Bacillus sp。）的pumilacidin样环状脂肽（CLP）。菌株176显着抑制溶藻弧菌的迁移能力和毒性。这里，为了进一步公开CLP抑制V的运动的机制。溶藻弧菌，我们首先应用转录组分析V。经或不经CLP处理的溶藻毒素。转录组结果显示，Na ⁺驱动的鞭毛运动中与细菌运动密切相关的几个重要成分的表达被显着抑制，表明Na ⁺的结构和功能CLP可能会禁用由鞭毛驱动的马达。蛋白质组间相互作用网络进一步分析了转录组数据，结果支持MotX（Na ⁺驱动的鞭毛运动的主要成分之一）最有可能成为CLP的作用靶标。结合基因敲除，电泳迁移率变动分析和免疫印迹技术，CLP被证明可通过与Na ⁺直接相互作用而影响溶藻弧菌鞭毛的旋转。鞭毛运动成分MotX，最终抑制细菌运动。有趣的是，发现MotX的同源物在不同的病原体中分布广泛且高度保守，这扩展了CLPs作为针对许多病原体中细菌运动性的抗菌药物的应用范围。