Ectomycorrhizae (ECMs) are a highly context‐dependent interactions that are not always beneficial for the plant host, sometimes leading to a decrease in plant growth. However, the molecular status of these plants remains unknown. We studied Populus × canescens microcuttings characterized by impaired growth in response to colonization by a Paxillus involutus strain via integrative proteomics–metabolomics analyses. The analysed strain was characterized by low compatibility and formed only mantles, not a Hartig net, in the majority of root tips. The increased abundance of photosynthetic proteins and foliar carbohydrates co‐occurred with signals of intensified resource exchange via the stems of colonized plants. In the roots, intensified C metabolism resulted in the biosynthesis of secondary C compounds unavailable to the fungal partner but also C skeletons necessary to increase insufficient N uptake from the hyphae. The stress response was also detected in colonized plants but was similar to that reported previously during mutualistic ECM interactions. In colonized poplar plants, mechanisms to prevent imbalanced C/N trade‐offs were activated. Root metabolism strongly depended on features of the whole plant, especially the foliar C/N budget. However, despite ECM‐triggered growth impairment and the foliar nutrient status, the fungal partner was recognized to be a symbiotic partner.

中文翻译：

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综合的蛋白质组学和代谢组学分析显示，被外生菌根真菌Paxillus involutus定植的杨××的分子调节，这限制了植物宿主的生长。

外生菌根（ECM）是高度依赖于上下文的交互作用，并不总是对植物宿主有益，有时会导致植物生长下降。但是，这些植物的分子状态仍然未知。我们研究了胡杨×canescens微切屑，这些切屑的特征在于对渐强线Paxillus involutus的定殖反应导致生长受损通过综合蛋白质组学-代谢组学分析获得菌株。所分析的菌株以低相容性为特征，并且在大多数根尖中仅形成地幔而不形成Hartig网。光合蛋白和叶碳水化合物的丰度增加与通过定植植物的茎进行的资源交换加剧的信号共同发生。在根部，强化的C代谢导致真菌伴侣无法获得次生C化合物的生物合成，而C骨架对于增加无法从菌丝吸收N所必需的。在定植的植物中也检测到了胁迫反应，但是与先前在相互的ECM相互作用中报道的相似。在殖民化的杨树植物中，激活了防止C / N平衡失衡的机制。根代谢很大程度上取决于整个植物的特性，尤其是叶面C / ​​N预算。但是，尽管ECM触发了生长障碍和叶片营养状态，但真菌伴侣被认为是共生伴侣。