Herein, Gold and magnetic particles (GoldMag), an enzyme mimetic of horseradish peroxidase (HRP), have been designed to construct a colorimetric sensor for cholesterol (Cho). The well-dispersed GoldMag was successfully prepared by green reduction using a self-assembly method based on the surface amino groups, and characterized by Fourier transform infrared (FTIR), X-Ray Photoelectron Spectroscopic (XPS) techniques. In the presence of H₂O₂, the resulting nanocomposites possessed enhanced intrinsic peroxidase-like activity and could catalytically oxidize 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) to produce a green colored product, which could be observed apparently by the naked eye. Based on the outstanding catalytic activity, the designed colorimetric sensor displayed a linear response for cholesterol in the range from 0.1 mg/mL to 7.5 mg/mL with a detection limit as low as 0.003 mg/mL. The proposed method was validated to determine cholesterol in real samples with satisfactory results.

在本文中，已经设计了辣根过氧化物酶（HRP）的酶模拟物金和磁性颗粒（GoldMag）来构建胆固醇（Cho）的比色传感器。通过基于表面氨基的自组装方法通过绿色还原成功地制备了分散良好的GoldMag，并通过傅里叶变换红外（FTIR），X射线光电子能谱（XPS）技术进行了表征。在H ₂ O ₂存在下，所得纳米复合材料具有增强的内在过氧化物酶样活性，并且可以催化氧化2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）以产生绿色产物，这可以通过观察明显地观察到。肉眼。基于出色的催化活性，设计的比色传感器对胆固醇的线性响应范围为0.1 mg / mL至7.5 mg / mL，检出限低至0.003 mg / mL。所提出的方法经过验证，可以测定真实样品中的胆固醇，结果令人满意。