当前位置: X-MOL 学术Sens. Actuators B Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Michael reaction-assisted fluorescent sensor for selective and one step determination of catechol via bifunctional Fe-MIL-88NH2 nanozyme
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.snb.2020.128547
Li Hou , Yuemei Qin , Tianran Lin , Ying Sun , Fanggui Ye , Shulin Zhao

The synthesized Fe-MIL-88NH2 was used as a nanozyme and fluorescence probe to successfully construct a selective fluorescent sensing platform for one step determination of catechol. Fe-MIL-88NH2 exhibited excellent peroxidase mimic property as well as fluorescence property. With the addition of H2O2 and catechol, Fe-MIL-88NH2 would catalyze the H2O2 to form hydroxyl radicals (OH). Then, the oxidation of catechol would be triggered by the formed OH, which resulted in the generation of 1, 2-benzoquinone. Afterwards, Michael reaction between the oxidation products of catechol (1, 2-benzoquinone) and Fe-MIL-88NH2 ligands was triggered and then quenched the fluorescence of Fe-MIL-88NH2. The principle of the selective determination of catechol was due to the assistant effect of Michael reaction. Based on the above sensing mechanism, a fluorescent sensing platform for the determination of catechol was established. The limit of detection was 0.0913 μmol L−1 (3S0, n=8/K) within a fast response of 10 min. This simple and selective strategy, based on the Michael reaction and the superiority of Fe-MIL-88NH2 nanozyme, provided a concise sensing platform for catechol determination, which broadened the scope of application of nanozyme and showed a great potential application for on-site testing in food and biology analysis.



中文翻译:

Michael反应辅助荧光传感器通过双功能Fe-MIL-88NH 2纳米酶选择性和一步测定邻苯二酚

合成的Fe-MIL-88NH 2用作纳米酶和荧光探针成功地构建了选择性荧光传感平台,用于一步测定邻苯二酚。Fe-MIL-88NH 2表现出优异的过氧化物酶模拟性质以及荧光性质。通过添加H 2 O 2和邻苯二酚,Fe-MIL-88NH 2将催化H 2 O 2形成羟基自由基(OH)。然后,形成的OH会引发邻苯二酚的氧化,从而导致生成1,2-苯并醌。之后,邻苯二酚(1,2-苯醌)的氧化产物与Fe-MIL-88NH 2之间的迈克尔反应触发配体,然后淬灭Fe-MIL-88NH 2的荧光。选择性测定儿茶酚的原理是由于迈克尔反应的辅助作用。基于上述检测机理,建立了测定儿茶酚的荧光检测平台。在10分钟的快速响应内,检出限为0.0913μmolL -1(3S 0,n = 8 / K)。这种简单,选择性的策略基于迈克尔反应和Fe-MIL-88NH 2纳米酶的优越性,为儿茶酚的测定提供了一个简洁的传感平台,拓宽了纳米酶的应用范围,显示了在现场的巨大应用潜力。食品和生物学分析中的测试。

更新日期:2020-07-06
down
wechat
bug