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Specific microbial diversity and functional gene (AOB amoA) analysis of a sponge-based aerobic nitrifying moving bed biofilm reactor exposed to typical pharmaceuticals.
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.scitotenv.2020.140660
Zi Song 1 , Xinbo Zhang 2 , Feiyun Sun 3 , Huu Hao Ngo 4 , Wenshan Guo 4 , Haitao Wen 2 , Chaocan Li 2 , Zumin Zhang 2
Affiliation  

Four bench-scale sponge-based aerobic nitrifying moving bed biofilm reactors (MBBRs) were used to treat municipal wastewater containing typical pharmaceuticals (1 mg/L, 2 mg/L and 5 mg/L). This preliminary research aims to investigate the effects of sulfadiazine (SDZ), ibuprofen (IBU) and carbamazepine (CBZ) on nitrification performance and explore specific microbial diversity and functional gene (Ammonia-oxidizing bacteria (AOB), amoA) of MBBRs. After 90 days of operation, the MBBR without pharmaceuticals could remove up to 97.4 ± 1.5% of NH4+-N while the removals of NH4+-N by the MBBRs with SDZ, IBU and CBZ were all suppressed to varying degrees. Based on the Shannon and Chao 1 index, the specific microbial diversity and richness in biofilm samples increased at a range of 1 mg/L to 2 mg/L pharmaceuticals (SDZ, IBU or CBZ) and started decreasing after the pharmaceutical concentration was higher than 2 mg/L. The determination of functional gene (AOB amoA) showed that Proteobacteria was the most dominant bacteria within all biofilms with the relative abundance ranging from 24.81% to 55.32%. Furthermore, Nitrosomonas was the most numerous genus in AOB, followed by Campylobacter and Thauera, whose relative abundance shifted under the pressure of different pharmaceuticals.



中文翻译:

暴露于典型药物的基于海绵的需氧硝化移动床生物膜反应器的特定微生物多样性和功能基因(AOB amoA)分析。

四个基于台式海绵的好氧硝化移动床生物膜反应器(MBBR)用于处理含有典型药物(1 mg / L,2 mg / L和5 mg / L)的市政废水。这项初步研究旨在研究磺胺嘧啶(SDZ),布洛芬(IBU)和卡马西平(CBZ)对硝化性能的影响,并探索MBBRs的特定微生物多样性和功能基因(氨氧化细菌(AOB),amoA)。手术90天后,不带药物的MBBR最多可去除97.4±1.5%的NH 4 + -N,而去除NH 4 +具有SDZ,IBU和CBZ的MBBR产生的-N均在不同程度上受到抑制。根据Shannon和Chao 1指数,生物膜样品中的特定微生物多样性和丰富度在1 mg / L至2 mg / L药物(SDZ,IBU或CBZ)的范围内增加,并且在药物浓度高于2毫克/升 功能基因(AOB amoA)的测定表明,变形杆菌是所有生物膜中最主要的细菌,相对丰度为24.81%至55.32%。此外,亚硝化单胞菌是AOB中最多的属,其次是弯曲杆菌属(Campylobacter)丘拉属(Thauera),它们的相对丰度在不同药物的压力下发生了变化。

更新日期:2020-07-01
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