Abstract Abiotic stresses are oxidative in nature and cause generation of reactive oxygen species (ROS) in plant bodies. Severe stresses can be harmful to the plant tissue, whereas sub-acute or lower doses of stresses could enhance or induce protective mechanisms, a biological phenomenon known as hormesis. The objective of this work was to examine the effect of hormetic as well as high doses of UV-B on the quality along with glucosinolate and hydroxy-cinnamate contents in broccoli florets during storage. An UV-B dose of 1.5 kJ m-2 was found to be hormetic from the color retention response. Color development, weight loss and respiration rate were monitored during 21 d of storage at 4 °C. The gene expression of dihomomethionine N-hydroxylase (CYP79F1), tryptophan N-hydroxylase 2 (CYP79B3), phenylalanine N-hydroxylase (CYP79A2), phenylalanine ammonia-lyase (PAL), chalcone synthase (CH) and flavanone 3-hydroxylase (F3H1) in the treated broccoli was also evaluated. The antioxidant capacity and the profiles of glucosinolates and hydroxy-cinnamates were determined for up to 14 d in broccoli florets stored at 4 °C by LC–MS. The hormetic dose of UV-B was effective in delaying the yellowing of broccoli florets. The initial respiration rate of the florets treated with the hormetic and a high dose (7.2 kJ m-2) was significantly high. The antioxidant capacity of florets was higher in UV-B treated florets relative to the control. The titers of indole-type glucosinolates and hydroxycinnamates in broccoli were significantly (p

中文翻译：

---

西兰花小花中的 UV-B 辐射刺激作用：储存期间小花中的硫代葡萄糖苷和羟基肉桂酸酯会增强 UV-B

摘要 非生物胁迫本质上是氧化性的，会导致植物体内产生活性氧（ROS）。严重的胁迫可能对植物组织有害，而亚急性或较低剂量的胁迫可以增强或诱导保护机制，这种生物现象称为兴奋作用。这项工作的目的是检查刺激性和高剂量 UV-B 对西兰花小花在储存期间的质量以及芥子油苷和羟基肉桂酸酯含量的影响。从保色反应中发现 1.5 kJ m-2 的 UV-B 剂量是刺激性的。在 4°C 下储存 21 天期间监测显色、重量损失和呼吸速率。二高甲硫氨酸N-羟化酶（CYP79F1）、色氨酸N-羟化酶2（CYP79B3）、苯丙氨酸N-羟化酶（CYP79A2）的基因表达，还评估了处理过的西兰花中的苯丙氨酸解氨酶 (PAL)、查耳酮合酶 (CH) 和黄烷酮 3-羟化酶 (F3H1)。通过 LC-MS 测定了在 4°C 下储存的西兰花小花中长达 14 天的抗氧化能力以及硫代葡萄糖苷和羟基肉桂酸酯的分布。UV-B 的刺激剂量可有效延缓西兰花小花的变黄。用兴奋剂和高剂量 (7.2 kJ m-2) 处理的小花的初始呼吸速率显着高。与对照相比，UV-B 处理的小花的抗氧化能力更高。西兰花中吲哚型硫代葡萄糖苷和羟基肉桂酸酯的滴度显着（p 通过 LC-MS 测定了在 4°C 下储存的西兰花小花中长达 14 天的抗氧化能力以及硫代葡萄糖苷和羟基肉桂酸酯的分布。UV-B 的刺激剂量可有效延缓西兰花小花的变黄。用兴奋剂和高剂量 (7.2 kJ m-2) 处理的小花的初始呼吸速率显着高。与对照相比，UV-B 处理的小花的抗氧化能力更高。西兰花中吲哚型硫代葡萄糖苷和羟基肉桂酸酯的滴度显着（p 通过 LC-MS 测定了在 4°C 下储存的西兰花小花中长达 14 天的抗氧化能力以及硫代葡萄糖苷和羟基肉桂酸酯的分布。UV-B 的刺激剂量可有效延缓西兰花小花的变黄。用兴奋剂和高剂量 (7.2 kJ m-2) 处理的小花的初始呼吸速率显着高。与对照相比，UV-B 处理的小花的抗氧化能力更高。西兰花中吲哚型硫代葡萄糖苷和羟基肉桂酸酯的滴度显着（p 2 kJ m-2) 非常高。与对照相比，UV-B 处理的小花的抗氧化能力更高。西兰花中吲哚型硫代葡萄糖苷和羟基肉桂酸酯的滴度显着（p 2 kJ m-2) 非常高。与对照相比，UV-B 处理的小花的抗氧化能力更高。西兰花中吲哚型硫代葡萄糖苷和羟基肉桂酸酯的滴度显着（p