Functional articular cartilage regeneration remains challenging, and it is essential to restore focal osteochondral defects and prevent secondary osteoarthritis. Combining autologous stem cells with therapeutic medical device, we developed a bi-compartmented implant that could promote both articular cartilage and subchondral bone regeneration. The first compartment based on therapeutic collagen associated with bone morphogenetic protein 2, provides structural support and promotes subchondral bone regeneration. The second compartment contains bone marrow-derived mesenchymal stem cell spheroids to support the regeneration of the articular cartilage. Six-month post-implantation, the regenerated articular cartilage surface was 3 times larger than that of untreated animals, and the regeneration of the osteochondral tissue occurred during the formation of hyaline-like cartilage. Our results demonstrate the positive impact of this combined advanced therapy medicinal product, meeting the needs of promising osteochondral regeneration in critical size articular defects in a large animal model combining not only therapeutic implant but also stem cells.

功能性关节软骨再生仍然具有挑战性，对恢复局灶性骨软骨缺损和预防继发性骨关节炎至关重要。将自体干细胞与治疗性医疗设备相结合，我们开发了一种双隔室植入物，可以促进关节软骨和软骨下骨的再生。基于与骨形态发生蛋白2相关的治疗性胶原蛋白的第一区室提供结构支持并促进软骨下骨再生。第二隔室包含骨髓来源的间充质干细胞球体，以支持关节软骨的再生。植入后六个月，再生的软骨表面比未治疗的动物大3倍，软骨样软骨形成过程中发生了软骨组织的再生。我们的结果证明了这种联合先进治疗药物的积极影响，满足了在不仅结合治疗性植入物而且还结合了干细胞的大型动物模型中，在关键尺寸的关节缺损中有望实现骨软骨再生的需求。