To investigate the role of bone marrow mesenchymal stem cell (BMSC)-derived exosomes in smoke inhalation lung injury. In this study, we initially isolated exosomes from BMSCs and identified them by western blot and transmission electron microscopy. BMSC-derived exosomes were then used to treat and models of smoke inhalation lung injury. Pathologic alterations in lung tissue, the levels of inflammatory factors and apoptosis-related factors, and the expression of HMGB1 and NF-κB were determined to evaluate the therapeutic effect of BMSC-derived exosomes. We found that BMSC-derived exosomes could alleviate the injury caused by smoke inhalation. Smoke inhalation increased the levels of inflammatory factors and apoptosis-related factors and the expression of HMGB1 and NF-κB, and these increases were reversed by BMSC-derived exosomes. HMGB1 overexpression abrogated the exosome-induced decreases in inflammatory factors, apoptosis-related factors and NF-κB. Collectively, these results indicate that BMSC-derived exosomes can effectively alleviate smoke inhalation lung injury by inhibiting the HMGB1/NF-κB pathway, suggesting that exosome, a noncellular therapy, is a potential therapeutic strategy for inhalation lung injury.

中文翻译：

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BMSC 衍生的外泌体通过阻断 HMGB1/NF-κB 通路减轻烟雾吸入性肺损伤

研究骨髓间充质干细胞（BMSC）来源的外泌体在烟雾吸入性肺损伤中的作用。在本研究中，我们首先从 BMSC 中分离出外泌体，并通过蛋白质印迹和透射电子显微镜对其进行鉴定。然后，骨髓间充质干细胞衍生的外泌体被用于治疗烟雾吸入性肺损伤并建立模型。测定肺组织的病理变化、炎症因子和凋亡相关因子的水平以及HMGB1和NF-κB的表达，以评估BMSC来源的外泌体的治疗效果。我们发现骨髓间充质干细胞来源的外泌体可以减轻吸入烟雾造成的损伤。烟雾吸入增加了炎症因子和凋亡相关因子的水平以及 HMGB1 和 NF-κB 的表达，并且这些增加被 BMSC 衍生的外泌体逆转。 HMGB1 过表达消除了外泌体诱导的炎症因子、凋亡相关因子和 NF-κB 的减少。总的来说，这些结果表明，BMSC来源的外泌体可以通过抑制HMGB1/NF-κB通路有效减轻烟雾吸入性肺损伤，这表明外泌体作为一种非细胞疗法，是吸入性肺损伤的潜在治疗策略。