Background

In this study, chromium (III) complex was synthesized from genistein (GEN) which had good hypoglycemic activity and inorganic chromium (III) element, and its hypoglycemic activity and sub-acute toxicity were studied.

Methods

The genistein-chromium (III) complex was synthesized by chelating chromium with genistein in ethanol and its structure was determined by LC–MS, atomic absorption spectroscopy, UV–vis spectroscopy, infrared spectroscopy, elemental and thermodynamic analysis. The anti-diabetic activity of the complex was assessed in db/db mice and C57 mice by daily oral gavage for 4 weeks. The sub-acute toxicity test was carried out on KM mice with this complex.

Results

The molecular structure of this complex was inferred as a complex [CrGEN₃] formed by three ligands and one chromium element. The complex could significantly improve the body weight of db/db mice, fasting blood glucose, random blood glucose, organ index, glycogen levels and the performance of OGTT (Oral Glucose Tolerance Test) and ITT (Insulin Tolerance Test) in db/db mice (p < 0.05). The morphology of liver, kidney, pancreas and skeletal muscle also had obviously improvement and repairment. Effects on serum indices and antioxidant enzymes activities of db/db mice showed that the serum profiles and antioxidant ability of complex group had significant improvement compared with the diabetic control group (p < 0.05 or p < 0.01), and some indices even returned to normal levels. In addition, this complex did not produce any hazardous symptoms or deaths in sub-acute toxicity test. High dose of [CrGEN₃] had no significant influence on serum indices and antioxidant capacity in normal mice, and the organ tissues maintained organized and integrity in the sub-acute toxicity study.

Conclusion

The study of the genistein-chromium (III) complex showed that the complex had good hypoglycemic activity in vivo, and did not have the potential toxicity. These results would provide an important reference for the development of functional hypoglycemic foods or pharmaceuticals.

中文翻译：

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db/db糖尿病小鼠中染料木素-铬（III）复合物的抗糖尿病特性及其在正常小鼠中的亚急性毒性评价。

背景

本研究以具有良好降糖活性的染料木黄酮(GEN)为原料,与无机铬(III)元素合成铬(III)配合物,对其降糖活性及亚急性毒性进行了研究。

方法

染料木素-铬 (III) 配合物是通过在乙醇中螯合铬与染料木黄酮合成的，其结构通过 LC-MS、原子吸收光谱、紫外-可见光谱、红外光谱、元素和热力学分析确定。通过每天口服强饲法在 db/db 小鼠和 C57 小鼠中评估复合物的抗糖尿病活性 4 周。用这种复合物对 KM 小鼠进行了亚急性毒性试验。

结果

该配合物的分子结构被推断为由三个配体和一个铬元素形成的配合物[CrGEN ₃ ]。该复合物可显着改善db/db小鼠的体重、空腹血糖、随机血糖、器官指数、糖原水平以及db/db小鼠的OGTT（口服葡萄糖耐受试验）和ITT（胰岛素耐受试验）的表现( p  < 0.05)。肝脏、肾脏、胰腺和骨骼肌的形态也有明显的改善和修复。对db/db小鼠血清指标和抗氧化酶活性的影响表明，与糖尿病对照组相比，复合物组的血清谱和抗氧化能力有显着改善（p  < 0.05或p < 0.01)，部分指标甚至恢复到正常水平。此外，该复合物在亚急性毒性试验中未产生任何危险症状或死亡。高剂量[CrGEN ₃ ]对正常小鼠血清指标和抗氧化能力无显着影响，亚急性毒性研究中器官组织保持组织完整。

结论

对染料木黄酮-铬(III)配合物的研究表明，该配合物在体内具有良好的降血糖活性，并且没有潜在的毒性。这些结果将为开发功能性降糖食品或药物提供重要参考。