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Enhancement the efficacy of spinosad for the control Sitophilus oryzae by combined application with diatomaceous earth and Trichoderma harzianum
Journal of Stored Products Research ( IF 2.7 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.jspr.2020.101663
Hassan A. Gad , Mohamed S. Al-Anany , Samir A.M. Abdelgaleil

Abstract Laboratory tests were carried out to evaluate the effect of diatomaceous earth (DE) originated from Egypt and Trichoderma harzianum on the efficacy of spinosad applied at low application rates against Sitophilus oryzae adults in stored wheat. Spinosad was assessed at 0.05, 0.1 and 0.5 mg/kg (ppm) alone or combined with DE at 100, 500 and 1000 mg/kg (ppm) or with T. harzianum at 3.3 × 106, 6.6 × 106 and 2.1 × 107 spores/kg. Adult mortality after 7, 14 and 21 days, progeny reduction after 45 and 90 days, and wheat weight loss were determined for all individual and binary treatments. Adult mortality increased with respect to increased exposure interval and application rate. Complete adult mortality (100%) was achieved after 21 days using 0.5 mg/kg of spinosad with all tested doses of DE and with 2.10 × 107 spores/kg of T. harzinum). Progeny production of S. oryzae was significantly reduced at all treatments compared to untreated wheat. No progeny was observed in the wheat treated with the combined treatments (0.5 mg/kg of spinosad + 1000 mg/kg of DE) and (0.5 mg/kg of spinosad + 2.10 × 107 spores/kg of T. harzinum) after 45 and 90 days. Similar trends were noted for wheat weight loss as the combined treatments at highest rates preserved the wheat intact and free from damage caused by S. oryzae for 90 days. Our findings suggest the combinations of spinosad at low rates with DE or T. harzianum can be effectively used for the control S. oryzae and provide long-term protection of stored wheat.

中文翻译:

硅藻土和哈茨木霉联合施用增强多杀菌素防治米粒线虫的药效

摘要 进行了实验室测试,以评估源自埃及和哈茨木霉的硅藻土 (DE) 对低施用率施用的多杀菌素对储存小麦中的米粒线虫成虫效果的影响。单独以 0.05、0.1 和 0.5 mg/kg (ppm) 或与 100、500 和 1000 mg/kg (ppm) 的 DE 或与 3.3 × 106、6.6 × 106 和 2.1 × 107 孢子的哈茨木霉联合评估多杀菌素/公斤。7、14 和 21 天后的成虫死亡率,45 天和 90 天后的后代减少,以及所有单独和二元处理的小麦体重减轻。成人死亡率随着接触间隔和施用率的增加而增加。使用 0.5 mg/kg 多杀菌素、所有测试剂量的 DE 和 2.10 × 107 个孢子/kg 哈津木霉在 21 天后达到完全成人死亡率 (100%)。与未处理的小麦相比,所有处理下米曲霉的后代产量均显着降低。在联合处理(0.5 mg/kg 多杀菌素 + 1000 mg/kg DE)和(0.5 mg/kg 多杀菌素 + 2.10 × 107 个孢子/kg 哈茨木霉)处理的小麦中未观察到后代 45 和90 天。小麦减重也有类似的趋势,因为最高比率的联合处理使小麦完好无损且不受米曲霉造成的损害 90 天。我们的研究结果表明,低剂量的多杀菌素与 DE 或 T. harzianum 的组合可有效用于控制米曲霉,并为储存的小麦提供长期保护。45 天和 90 天后,5 mg/kg 多杀菌素 + 1000 mg/kg DE)和(0.5 mg/kg 多杀菌素 + 2.10 × 107 个孢子/kg 哈茨木霉)。小麦减重也有类似的趋势,因为最高比率的联合处理使小麦完好无损且不受米曲霉造成的损害 90 天。我们的研究结果表明,低剂量的多杀菌素与 DE 或 T. harzianum 的组合可有效用于控制米曲霉,并为储存的小麦提供长期保护。45 天和 90 天后,5 mg/kg 多杀菌素 + 1000 mg/kg DE)和(0.5 mg/kg 多杀菌素 + 2.10 × 107 个孢子/kg 哈茨木霉)。小麦减重也有类似的趋势,因为最高比率的联合处理使小麦完好无损且不受米曲霉造成的损害 90 天。我们的研究结果表明,低剂量的多杀菌素与 DE 或 T. harzianum 的组合可有效用于控制米曲霉,并为储存的小麦提供长期保护。
更新日期:2020-09-01
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