Accurate determination of Sarcosine (SAR) in urine with high sensitivity and selectivity is important, because it was recently recommended as a prospective biomarker for prostate cancer (PCa) and significant for the early identification of PCa. In this study, an electrochemical sensor based on Fe₃O₄ incorporated metal–organic frameworks (MOFs) @molecularly imprinted polymer (MIP) was constructed for SAR detection. Magnetic Fe₃O₄ nanoparticles embedded zeolitic imidazolate framework-8 (ZIF-8) was used as the support of MIP. MIP provides specific recognition sites for template molecules SAR and MOFs increase the rate of mass transfer and adsorption capacity due to the porous structure. The synthesized super-magnetic Fe₃O₄@ZIF-8@MIP was self-assembled onto an Au electrode in magnetic field and used as the sensing unit of electrochemical sensor. Cyclic voltammetry was used to monitor the electrochemical behavior, and the binding of SAR resulted in a reduction in the measured current. The results revealed a wide linear range from 1 to 100 pM towards trace SAR determination, with extremely low limit of detection down to 0.4 pM. In conclusion, the Fe₃O₄@ZIF-8@MIP based sensor provides a selective, sensitive, and convenient method for SAR diagnosis and other cancer marker detection.

准确测定尿液中的肌氨酸（SAR）具有很高的灵敏度和选择性非常重要，因为最近推荐将其作为前列腺癌（PCa）的前瞻性生物标志物，对PCa的早期识别具有重要意义。在这项研究中，构建了一种基于Fe ₃ O ₄结合金属-有机骨架（MOF）@分子印迹聚合物（MIP）的电化学传感器，用于SAR检测。磁性Fe ₃ O ₄纳米粒子嵌入的沸石咪唑盐骨架8（ZIF-8）被用作MIP的载体。MIP为模板分子SAR提供了特定的识别位点，而MOF由于具有多孔结构而提高了传质速率和吸附能力。合成超磁性Fe ₃O ₄ @ ZIF-8 @ MIP在磁场中自组装到Au电极上，用作电化学传感器的传感单元。循环伏安法用于监测电化学行为，SAR的结合导致测量电流的减少。结果表明，从1到100 pM的线性范围很宽，可以测定痕量SAR，检测限极低，低至0.4 pM。总之，基于Fe ₃ O ₄ @ ZIF-8 @ MIP的传感器为SAR诊断和其他癌症标志物检测提供了一种选择性，灵敏和方便的方法。