This study involves continuous co-digestion of swine manure and pineapple waste mixture using two-stage anaerobic reactors and examines hydraulic retention time (HRT) and substrate heat pre-treatment. The maximum hydrogen and methane production rates of 1488.62 and 991.57 mL/L/d, respectively, reached optimal HRTs of 4.5 h in the hydrogen production fermenter (HPF) and 9 d in the methane production fermenter (MPF) using heat pre-treatment. Acetic acid is a dominant volatile fatty acid of the soluble metabolites with values 70%–73% under all the tested conditions and increased values under heat pre-treatment and high HRT. Firmicutes and Euryarchaeota are the main bacteria species detected in HPF and MPF, respectively. The optimal total energy of 196.47 kJ/L/d and chemical oxygen demand (COD) removal efficiency of 90% were obtained by a complete anaerobic co-digestion process at a high substrate concentration of 105 g COD/L and low HRT of 4.5 h. This shows that the two-stage co-digestion process could increase the COD removal efficiency, hydrogen production rate, and net energy gains and produce high quality biogas and significantly reduce fermentation time.

这项研究涉及使用两级厌氧反应器连续共同消化猪粪和菠萝废物混合物，并检查水力停留时间（HRT）和基质热处理。使用制热预处理，在制氢发酵罐（HPF）中，最大氢气和甲烷生产率分别达到1488.62和991.57 mL / L / d，达到最佳HRT为4.5 h，在制甲烷发酵罐（MPF）中达到9 d。乙酸是可溶性代谢产物的主要挥发性脂肪酸，在所有测试条件下其值均在70％至73％之间，而在热预处理和高HRT下其值则在增加。Firmicutes和Euryarchaeota是分别在HPF和MPF中检测到的主要细菌种类。在105 g COD / L的高底物浓度和4.5 h的低HRT的条件下，通过完整的厌氧共消化工艺，可获得196.47 kJ / L / d的最佳总能量和90％的化学需氧量（COD）去除效率。 。这表明两阶段共消化过程可以提高COD去除效率，制氢率和净能量收益，并产生高质量的沼气，并显着减少发酵时间。