Salidroside and rosavins have been found as the most potent ingredients used in Rhodiola based herbal formulations. Rhodiola imbricata is a medicinal herb of the trans-Himalayan Ladakh region of India. Since, the natural supply of this herb is rapidly decreasing, due to its over-exploitation, high altitude region, and traditional usage in the Amchi system of medicine. In the present study, friable callus culture was developed for the first time from leaf and root explants of R. imbricata. Furthermore, callus cell lines were also evaluated for rapid growth rate and high metabolite accumulation. The results revealed that callus induction frequency observed in juvenile leaves (100 %) and roots (87.50 %) in MS medium enriched with 0.5 mg/L TDZ and 1 mg/L NAA. Scanning electron microscopy (SEM) analysis was done for histological observations of the callus surface. A selective and efficient ultra-performance liquid chromatography with PDA detector (UPLC-PDA) method was developed and validated for the quantification of phenylethanoids, phenylpropanoids, and phenolic acids. The maximum salidroside was detected in leaf derived friable green calli (3.59 mg/g DW) subsequently followed by leaf derived friable white calli (2.31 mg/g DW). While rosavin and rosarin were detected maximum in root derived compact green calli (0.15 mg/g DW) and root derived friable green calli (0.07 mg/g DW). Genes encoding enzymes involved in salidroside and rosavins biosynthesis were also investigated for transcript abundance in wild as well as in vitro cultures using the RT-qPCR approach. The present study explained the friable callus culture as a potential alternative approach to obtain higher metabolite yield in R. imbricata. Further, it can be used for sequential scale-up of metabolite at bioreactor level to meet the industrial demand.

红景天苷和rosavins被发现是基于红景天的草药配方中使用的最有效成分。红景天是印度反喜马拉雅山拉达克地区的草药。由于这种草药的过度开发，高海拔地区以及Amchi医药系统的传统用法，其天然供应量正在迅速减少。在本研究中，脆性愈伤组织培养是首次从圆根罗非鱼的叶和根外植体发展而来。。此外，还评估了愈伤组织细胞系的快速生长速率和高代谢产物积累。结果表明，在富含0.5 mg / L TDZ和1 mg / L NAA的MS培养基中，幼叶（100％）和根（87.50％）中观察到愈伤组织诱导频率。进行了扫描电子显微镜（SEM）分析，以观察愈伤组织表面的组织学现象。研发了一种具有PDA检测器（UPLC-PDA）的高效选择性超高效液相色谱法，并已用于定量苯乙类固醇，苯丙烷类和酚酸的定量分析。在源自叶的脆性绿色愈伤组织（3.59 mg / g DW）中检测到最大的红景天苷，随后是源自叶的脆性白色愈伤组织（2.31 mg / g DW）。虽然在根衍生的紧凑型绿色愈伤组织中检测到了罗沙文和罗沙林的最大值（0。15 mg / g DW）和根衍生的脆性绿色愈伤组织（0.07 mg / g DW）。还研究了与红景天苷和rosavins生物合成有关的编码酶的基因在野生和野生情况下的转录丰度。使用RT-qPCR方法进行体外培养。本研究解释了脆弱的愈伤组织培养作为一种潜在的替代方法，可以提高圆纹夜蛾的代谢产物产量。此外，它可用于在生物反应器水平上按顺序放大代谢产物以满足工业需求。