Leaves of Hedera helix L. have been widely used for the treatment of productive cough and chronic inflammatory bronchitis. The pharmacological effect may be caused by the presence of many active compounds which also may show additively or synergistically action. The quality of raw material, as well as, the pharmacological activity strongly depends on the phytochemical composition thus, phytoprofiling and phytochemical markers identification are essential for the standardization of herbal materials for comprehensive characterization of herbal drugs. The aim of this comparative investigation was to determine the composition of flavonoids, phenolic acids, triterpene saponins, amino acids, and individual fast-acting antioxidants in H. helix leaf samples collected from different climatic and environmental regions of Europe. The determined predominant phenolic compounds and radical scavengers were 3,5-caffeoylquinic acid and chlorogenic acid corresponding up to 80 % of total radical scavenging activity. These compounds could be chosen as analytical markers. The results have revealed two major phytogeographical groups with characteristic phytochemical composition: the central part and the southern part growing sites, with latter having significantly lower amounts of all compounds tested. The determined patterns of bioactive compounds suggest distinct chemotypes with characteristic phenolic acid, flavonoid, triterpene saponin, and amino acid complexes. Developed and validated analytical methods are suitable for fingerprint profiling of the phenolic, radical scavenging, triterpenic compounds, and amino acids and marker elucidation for standardization.

常春藤叶片已被广泛用于治疗生产性咳嗽和慢性炎性支气管炎。药理作用可能是由于存在许多活性化合物而引起的，这些活性化合物也可能表现出加和或协同作用。原料的质量以及药理活性在很大程度上取决于植物化学成分，因此，植物概况分析和植物化学标记物的鉴定对于草本材料的标准化，对于草本药物的全面表征至关重要。该比较研究的目的是要确定类黄酮，酚酸，三萜皂甙，氨基酸在组合物中，和个人速效抗氧化剂H.螺旋从欧洲不同气候和环境区域收集的叶子样本。所确定的主要酚类化合物和自由基清除剂是3,5-咖啡酰奎尼酸和绿原酸，相当于总自由基清除活性的80％。这些化合物可以选择作为分析标记。结果表明，有两个主要的植物地理组别具有特征性的植物化学组成：中部和南部生长地点，而后者的所有受测化合物的含量均明显较低。确定的生物活性化合物模式表明具有特征性酚酸，类黄酮，三萜皂苷和氨基酸复合物的不同化学型。开发和验证的分析方法适用于酚醛，自由基清除剂，