Perfluorinated compounds (PFCs) contamination of soil has attracted global attention in recent years but influences of PFCs on microorganisms in the soil environment have not been fully described. In this study, the effects of perfluorooctane sulphonate (PFOS) and perfluoroctanoic acid (PFOA) on bacterial communities were determined by Illumina Miseq sequencing and Illumina Hiseq Xten. The stimulation of PFCs pollutants on soil bacterial richness and community diversity were observed. Sequencing information indicated that Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Firmicutes, and Gemmatimonadetes were the dominant bacterial phyla. Two genera, Bacillus and Sphingomonas, exhibited adverse responses toward PFCs pollution. Carbohydrate-active enzymes (CAZy), Kyoto Encyclopedia of Genes and Genomes (KEGG) and NCBI databases were used to elucidate the proteins and function action of soil microbial to PFCs pollution. Pathways such as Carbohydrate metabolism, Global and overview maps and Membrane transport in the soil microbes were affected by PFCs stress. CAZy analysis revealed that glycosyl transferases (GTs) in PFCs-polluted soils showed more active, while glycoside hydrolases (GHs) were inhibited severely.

近年来，土壤中的全氟化合物（PFCs）污染已引起全球关注，但尚未完全描述PFCs对土壤环境中微生物的影响。在这项研究中，通过Illumina Miseq测序和Illumina Hiseq Xten确定了全氟辛烷磺酸盐（PFOS）和全氟辛酸（PFOA）对细菌群落的影响。观察到PFCs污染物对土壤细菌丰富度和群落多样性的刺激作用。排序信息表明，变形菌门，酸杆菌门，放线菌，绿弯菌门，厚壁菌门，和芽单胞菌门是占主导地位的细菌门。两个属，芽孢杆菌和鞘氨醇单胞菌，表现出对全氟化碳污染的不良反应。碳水化合物活性酶（CAZy），《京都基因与基因组百科全书》（KEGG）和NCBI数据库用于阐明蛋白质和土壤微生物对PFCs污染的作用。PFCs应力会影响碳水化合物代谢，全局和总览图以及膜在土壤微生物中的迁移等途径。CAZy分析表明，PFCs污染土壤中的糖基转移酶（GTs）活性更高，而糖苷水解酶（GHs）则受到严重抑制。