To reveal nitrogen removal mechanisms under environmental stresses, biofilm reactors were operated at different temperatures (10 °C–35 °C) treating saline wastewater (salinity 3%). The results showed nitrogen removal efficiency was 98.46% at 30 °C and 60.85% at 10 °C, respectively. Both ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) participated in nitrification. 94.9% of the overall ammonia oxidation was attributed to AOA at 10 °C, but only 48.2% of that was undertaken by AOA at 35 °C. AOA had a greater contribution at low temperature, which demonstrated that nitrogen removal pathway varied with temperature. Aerobic denitrification was more stable than anoxic denitrification. High-throughput sequencing showed Crenarchaeota was the dominant AOA (97.02–34.47%), cooperating with various heterotrophic AOB. Real-time PCR indicated that AOA was three orders of magnitude more abundant than AOB. AOA was more resistant to low temperature and high-saline stresses. Ammonia oxidizers had distinct responses to temperature change and showed diverse relationships at different temperatures.

为了揭示在环境压力下的脱氮机理，生物膜反应器在不同温度（10°C–35°C）下处理含盐废水（盐度3％）。结果表明，脱氮效率在30°C下为98.46％，在10°C下为60.85％。氨氧化古细菌（AOA）和氨氧化细菌（AOB）均参与硝化作用。在10°C时，总氨氧化的94.9％归因于AOA，但在35°C时，只有48.2％由AOA氧化。低温下AOA的贡献更大，这表明脱氮途径随温度的变化而变化。好氧反硝化比缺氧反硝化更稳定。高通量测序显示Crenarchaeota是主要的AOA（97.02–34.47％），与各种异养型AOB合作。实时PCR表明，AOA比AOB丰富了三个数量级。AOA更耐低温和高盐胁迫。氨氧化剂对温度变化具有不同的响应，并在不同温度下表现出多种关系。