The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10⁻¹⁰ g mL⁻¹ (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL⁻¹. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

具有高度传染性的诺如病毒（NoV）是急性胃肠炎的最常见病原体，每年在全世界导致200,000多人死亡。快速而灵敏的检测方法是有效预防和及时识别NoV污染的前提。在本研究中，我们开发了一种光电化学（PEC）生物传感器，并与新型定制单克隆抗体（mAb）结合用于特异性和灵敏的NoV检测。我们的系统可以在30分钟内以浓度依赖的方式检测到重组NoV衣壳蛋白VP1的水平，低至2×10 ^-10 g mL ^-1（4.9 pM）。更重要的是，该生物传感器具有多种功能，可检测低至46拷贝μL ^-1的真实样品中分离出的病毒。这些发现表明，该系统有可能用作诊断NoV感染和检测受NoV污染的食品样本的便捷即时服务系统。