Abstract In this work is presented the synthesis of the inclusion complexes of amygdalin with β-cyclodextrin by three methods: kneading, co-precipitation and freeze drying and their characterization. The inclusion compounds were synthesized using a molar ratio of amygdalin: β-cyclodextrin of 1:1. Synthesized compounds were analyzed by FTIR spectroscopy, X-rays, thermal analysis and the results confirm the formation of inclusion compounds by amygdalin with β-cyclodextrin. The studies carried out have shown the protective effect of amygdalin-β-cyclodextrin compounds obtained by freeze drying, kneading and coprecipitation against free radicals (SOD-like activity in vivo) and the results of in vitro cell cytotoxicity of the compounds on HeLa cell line compared to pure amygdalin. Compound obtained by freeze drying has the best SOD-like activity and cytotoxicity on HeLa tumor cells, so it may be considered as potential therapeutic agent.

中文翻译：

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具有β-环糊精和sod样活性的苦杏仁苷包合物的合成、表征以及对hela肿瘤细胞的细胞毒性

摘要 本文介绍了通过捏合、共沉淀和冷冻干燥三种方法合成苦杏仁苷与β-环糊精的包合物及其表征。使用 1:1 的苦杏仁苷: β-环糊精的摩尔比合成包合物。合成的化合物通过 FTIR 光谱、X 射线、热分析进行分析，结果证实苦杏仁苷与 β-环糊精形成包合物。开展的研究表明，通过冷冻干燥、揉捏和共沉淀获得的苦杏仁苷-β-环糊精化合物对自由基的保护作用（体内SOD样活性）以及这些化合物对HeLa细胞系的体外细胞毒性结果与纯苦杏仁苷相比。