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Nutrient-conditioned intake stimulation does not require a distinctive flavor cue in rats
Appetite ( IF 4.6 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.appet.2020.104793
Anthony Sclafani 1 , Karen Ackroff 1
Affiliation  

The postoral actions of nutrients in rodents can stimulate intake and condition flavor preferences through an appetition process. Appetition is revealed in rodents by their increased intake of and preference for a flavored solution paired with intragastric (IG) nutrient infusions. Here we determined if IG 16% maltodextrin (MD) infusions can stimulate intake and preference in the absence of a distinctive flavor cue. Rats implanted with IG catheters were given chow and water 2 h/day followed, 2 h later, by 20-h oral access to water paired with IG MD infusions. Other rats were given bitter sucrose octaacetate solution (SOA) paired with IG MD infusions 20 h/day. Over 8 test days, the SOA rats increased their total 20-h fluid intake (oral + IG) from 26 to 119 g/20 h and Water rats increased their intake from 31 to 96 g/20 h. When infused IG with water instead of MD in a 4-day extinction test, the SOA and Water groups reduced their fluid intakes to 45-48 g/20 h. When oral fluids were again paired with IG MD infusions, the SOA and Water groups increased their intakes to 115 and 109 g/20 h, respectively. In two-bottle tests, the SOA rats drank more SOA paired with IG MD than water paired with IG water. Water rats given the choice of a water bottle paired with IG MD and water bottle paired with IG water did not consistently prefer the H2O/ID MD bottle. Instead they displayed side or sipper tube preferences although neither cue was consistently paired with IG MD during one-bottle training.

中文翻译:

营养条件摄入刺激不需要大鼠的独特风味提示

啮齿动物中营养素的后作用可以通过食欲过程刺激摄入和调节风味偏好。啮齿动物的食欲是通过增加对调味溶液的摄入量和偏好与胃内 (IG) 营养输注配对来显示的。在这里,我们确定了 IG 16% 麦芽糊精 (MD) 输注是否可以在没有独特风味提示的情况下刺激摄入和偏好。植入 IG 导管的大鼠每天 2 小时喂食食物和水,然后 2 小时后,通过 20 小时口服水与 IG MD 输注配对。其他大鼠被给予苦味蔗糖八乙酸酯溶液 (SOA) 与 IG MD 输注配对,20 小时/天。在 8 天的测试中,SOA 大鼠的 20 小时液体总摄入量(口服 + IG)从 26 增加到 119 克/20 小时,而水大鼠的摄入量从 31 增加到 96 克/20 小时。在为期 4 天的消光试验中,当给 IG 注入水而不是 MD 时,SOA 和水组将其液体摄入量减少到 45-48 克/20 小时。当口服液再次与 IG MD 输注配对时,SOA 和水组的摄入量分别增加到 115 和 109 克/20 小时。在两瓶测试中,SOA 大鼠喝了更多的 SOA 与 IG MD 配对,而不是与 IG 水配对的水。水鼠可以选择与 IG MD 配对的水瓶,而与 IG 水配对的水瓶则并不总是喜欢 H2O/ID MD 瓶。相反,他们显示了侧管或吸管偏好,尽管在单瓶训练期间,这两种提示都没有始终与 IG MD 配对。SOA 和水组的摄入量分别增加到 115 和 109 克/20 小时。在两瓶测试中,SOA 大鼠喝了更多的 SOA 与 IG MD 配对,而不是与 IG 水配对的水。水鼠可以选择与 IG MD 配对的水瓶,而与 IG 水配对的水瓶则并不总是喜欢 H2O/ID MD 瓶。相反,他们显示了侧管或吸管偏好,尽管在单瓶训练期间,这两种提示都没有始终与 IG MD 配对。SOA 和水组的摄入量分别增加到 115 和 109 克/20 小时。在两瓶测试中,SOA 大鼠喝了更多的 SOA 与 IG MD 配对,而不是与 IG 水配对的水。水鼠可以选择与 IG MD 配对的水瓶,而与 IG 水配对的水瓶则并不总是喜欢 H2O/ID MD 瓶。相反,他们显示了侧管或吸管偏好,尽管在单瓶训练期间,这两种提示都没有始终与 IG MD 配对。
更新日期:2020-11-01
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