Abstract

Conditions for the extraction of a phosphonylated nonapeptide, phosphonylated tyrosine, and cyclohexylmethylphosphonic acid from human blood plasma followed by HPLC determination with detection by high-resolution tandem mass spectrometry were optimized. Conditions for human blood plasma sample preparation, mass-spectrometric detection in selected reaction monitoring mode, and gradient elution were chosen to determine the above markers of exposure to cyclohexylmethylfluorophosphonate. The detection limit of the initial substance introduced into blood plasma was 0.05–0.1 ng/mL depending on the test marker.

中文翻译：

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高分辨率液相色谱-质谱法测定人血浆中的环己基甲基氟膦酸酯代谢物

摘要

优化了从人血浆中提取膦酰化九肽，膦酰化酪氨酸和环己基甲基膦酸，然后通过高效液相色谱法测定和高分辨率串联质谱检测的条件。选择用于人血浆样品制备，在选定的反应监测模式下进行质谱检测以及梯度洗脱的条件，以确定上述暴露于环己基甲基氟膦酸酯的标志物。根据测试标记物，引入血浆的初始物质的检出限为0.05–0.1 ng / mL。