At LFB USA, Inc., the ultimate use for transgenic cloned goats is for the production of recombinant human protein therapeutics in their milk. This retrospective analysis of the Somatic Cell Nuclear Transfer (SCNT) program, spanning from 1998 to 2010, examined parameters potentially affecting the outcomes and efficiencies in this commercial operation. Over 37,000 + ova were utilized in the SCNT protocol producing a total of 203 cloned goats. Fifty one (51) clones were produced from non-transfected (transgenic and non-transgenic animal donor) cell lines and 152 clones were produced from transfected cell lines. Comparisons and summaries of (a) transfected versus non-transfected cell lines, (b) relationship of SCNT parameters to offspring produced, (c) skin versus fetal cells, (d) fresh versus cryopreserved cells, (e) parameters from all cell lines used versus those producing SCNT offspring, (f) variation among cell sources, (g) methods of SCNT parturition management and effects on live offspring, and lastly (h) SCNT variation by program are reported. Findings indicate that (a) non-transfected cell lines were more efficient versus transfected cell lines in generating viable cloned offspring on a per reconstructed embryo transferred basis, (b) transfected fetal fibroblasts had improved efficiency versus transfected skin fibroblasts, (c) the percentage of non-transfected cell lines that produced offspring was statistically higher than transfected cell lines, (d) and induction of parturition improved the percentage of viable offspring. In summary, this retrospective analysis on the SCNT process has identified certain parameters for improved efficiency in producing viable cloned goats in a commercial setting.

在LFB USA，Inc.，转基因克隆山羊的最终用途是在其乳汁中生产重组人蛋白质治疗剂。这项对1998年至2010年间的体细胞核移植（SCNT）计划的回顾性分析研究了可能影响这一商业运营的结果和效率的参数。在SCNT协议中使用了超过37,000 +的卵，总共生产了203只克隆山羊。从未转染的（转基因和非转基因动物供体）细胞系产生了五十一个（51）克隆，并且从转染的细胞系产生了152个克隆。（a）转染和未转染细胞系的比较和总结，（b）SCNT参数与产生的后代的关系，（c）皮肤与胎儿细胞，（d）新鲜与冷冻保存的细胞，（e）使用的所有细胞系的参数与产生SCNT的后代的参数；（f）细胞来源之间的变异；（g）SCNT的分娩管理方法以及对活后代的影响；最后（h）报告了程序产生的SCNT变异。研究结果表明，（a）未转染的细胞系比转染的细胞系在每个重建的胚胎移植基础上生成活的克隆后代的效率更高，（b）转染的胎儿成纤维细胞比转染的皮肤成纤维细胞具有更高的效率，（c）百分比产生后代的未转染细胞系的统计学上高于转染的细胞系，（d），分娩诱导提高了存活后代的百分比。综上所述，