The objective of the present study was to establish the most suitable culture medium for the isolation of H. pylori from environmental and clinical samples. Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated with serial dilutions of H. pylori NCTC 11637 strain at a final concentration of 10⁴ and 10³ CFU/ml and the recovery rates were calculated. From inoculated tap water and wastewater samples, H. pylori colonies were recovered from four out of the analyzed culture media. When fecal samples were analyzed, the isolation of the pathogen under study was only possible from two culture media. Different optimal media were observed for each type of sample, even for wastewater and stool samples. Nevertheless, our results indicated that the combination of Dent Agar with polymyxin B sulfate did not inhibit the growth of H. pylori and was highly selective for its recovery, regardless of the sample origin. Thus, we propose the use of this medium as a diagnostic tool for the isolation of H. pylori from environmental and clinical samples, as well as for epidemiological studies.

本研究的目的是建立最合适的培养基，用于从环境和临床样品中分离幽门螺杆菌。比较和评估了十种不同的培养基。其中四个已在前面进行了描述，并在本研究中进行了修改。其余媒体是从头设计的。用连续稀释的幽门螺杆菌NCTC 11637菌株以终浓度10 ⁴和10 ³  CFU / ml接种三种不同的基质（自来水，废水和粪便），并计算回收率。从接种的自来水和废水样本中获得幽门螺杆菌从四种分析培养基中回收菌落。当分析粪便样品时，只能从两种培养基中分离出所研究的病原体。对于每种类型的样品，甚至对于废水和粪便样品，都观察到不同的最佳培养基。然而，我们的结果表明，Dent Agar与多粘菌素B硫酸盐的结合不会抑制幽门螺杆菌的生长，并且对回收它具有高度选择性，而与样品来源无关。因此，我们建议将该介质用作从环境和临床样品中分离幽门螺杆菌以及进行流行病学研究的诊断工具。