Microbial enzymes are the safe alternatives to chemical based bleaching of pulp in paper mills. For effective biobleaching, both hemicellulolytic and lignolytic enzymes are required. This study reports laccase (L) + xylanase (X) and laccase (L) + mannanase (M) enzyme concoctions for pulp biobleaching derived from Bacillus sp. LX and Bacillus sp. LM isolated from the decaying organic matter. All enzymes were thermo-alkali-stable, hence were suitable for their application in pulp biobleaching. When a mixture of L + X/L + M was used for mixedwood pulp biobleaching, 46.32/40.25% reduction in kappa number; 13.21/10.01% and 3.36/2.76% improvement in brightness and whiteness was achieved respectively. Moreover, no laccase mediator system was required in the current process. Significant changes in the structure of enzymatically treated pulp were also observed. All these properties make these concoctions of enzymes suitable for their application in pulp and paper mill.

中文翻译：

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用于造纸的混合木浆与木质素分解酶和半纤维素分解酶的组合生物漂白。

微生物酶是造纸厂纸浆化学漂白的安全替代品。对于有效的生物漂白，需要半纤维素分解酶和木质素分解酶。本研究报告了漆酶 (L) + 木聚糖酶 (X) 和漆酶 (L) + 甘露聚糖酶 (M) 酶混合物，用于来自芽孢杆菌的纸浆生物漂白。LX和芽孢杆菌sp. LM 从腐烂的有机物质中分离出来。所有酶都是热碱稳定的，因此适用于它们在纸浆生物漂白中的应用。当L+X/L+M的混合物用于混合木浆生物漂白时，卡伯值降低46.32/40.25%；亮度和白度分别提高了 13.21/10.01% 和 3.36/2.76%。此外，目前的工艺不需要漆酶介体系统。还观察到酶处理纸浆结构的显着变化。所有这些特性使这些酶混合物适用于它们在纸浆和造纸厂中的应用。