The extent of mycorrhizal fungi colonization is an important factor for determining the function of mycorrhizal fungi in fungi–host interaction, and quantifying the extent of mycorrhizal fungi colonization is a fundamental and essential task for researchers engaged in mycorrhizal studies. Intersect methods, such as the gridline intersect and magnified intersections methods, are accurate and objective, and are widely used to assess the colonization status of arbuscular mycorrhizal (AM) fungus. However, no convenient procedures or add-ins for Excel spreadsheets have been developed to simplify these methods. Here, we propose a procedure using the Visual Basic for Application (VBA) program in Excel that is based on the magnified intersections method, which we refer to as VBA–AMF (arbuscular mycorrhizal fungi). Time-saving and convenience are the two most prominent advantages of the VBA–AMF procedure, as it enables researchers to compute the colonization rate of AM fungi in roots, and consequently the extent of root colonization by AM fungi. VBA–AMF can also be modified to measure the status of other fungal colonizations in plant roots following the same strategy.

中文翻译：

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VBA-AMF：基于放大交集法的 VBA 程序，用于定量记录丛枝菌根真菌的根定殖。


菌根真菌定植程度是决定菌根真菌在真菌与宿主相互作用中功能的重要因素，而量化菌根真菌定植程度是从事菌根研究的研究者的一项基础性和必要性任务。交叉法，如网格线交叉法和放大交叉法，准确、客观，广泛用于评估丛枝菌根（AM）真菌的定植状况。然而，尚未开发出适用于 Excel 电子表格的方便程序或插件来简化这些方法。在这里，我们提出了一个使用 Excel 中的 Visual Basic for Application (VBA) 程序的过程，该程序基于放大交叉法，我们将其称为 VBA-AMF（丛枝菌根真菌）。省时和方便是 VBA-AMF 程序的两个最突出的优点，因为它使研究人员能够计算 AM 真菌在根部的定植率，从而计算 AM 真菌在根部定植的程度。还可以修改 VBA-AMF，以按照相同的策略测量植物根部其他真菌定植的状态。