The molecular detection of Norovirus GI and Norovirus GII in the Tunisian industrial wastewater treatment plant of Charguia I was conducted to test the effectiveness of secondary biological treatment using the activated sludge procedure and the UV-C₂₅₄ tertiary treatment radiation using a UV disinfection prototype to upgrade the quality of the purified wastewater. A total of 140 sewage samples were collected from the two lines of sewage treatment procedures. Norovirus GI and Norovirus GII have been found and quantified using Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) in 66.4 and 86.4% of the collected wastewater samples. The Norovirus GI and GII mean concentration values got in the treated effluents showed a significant decrease of Norovirus viral content rates detected from upstream to downstream of activated sludge procedures and at the output of UV-C₂₅₄ treatment. These findings characterise and denote for the first time the effectiveness of biological and UV-C254 treatment for Norovirus GI and Norovirus GII removal in Tunis City, northern Tunisia. Also, these data underlined the fact that purified sewage makes up a route of gastroenteritis virus transmission and a cause of viral gastroenteritis associated with water-borne and food-borne outbreaks.

中文翻译：

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突尼斯工业废水处理厂中活性污泥处理程序和UV-C254在诺如病毒灭活中的有效性。

在Charguia I的突尼斯工业废水处理厂中进行了诺如病毒GI和诺如病毒GII的分子检测，以测试采用活性污泥法和UV-C ₂₅₄进行二次生物处理的有效性使用UV消毒原型进行第三次处理辐射，以提高纯化废水的质量。两条污水处理程序共收集了140个污水样品。使用实时逆转录聚合酶链反应（qRT-PCR）在收集的66.4％和86.4％的废水样本中发现并定量了诺如病毒GI和诺如病毒GII。在处理后的废水中获得的诺如病毒GI和GII平均浓度值显示，从活性污泥程序的上游到下游以及在UV-C ₂₅₄的输出中检测到的诺如病毒的病毒含量率显着降低治疗。这些发现表征并首次表明了在突尼斯北部突尼斯市进行生物和UV-C254治疗诺如病毒GI和诺如病毒GII去除的有效性。同样，这些数据强调了这样一个事实，即纯净的污水构成了胃肠炎病毒的传播途径，并且是与水源性和食源性暴发有关的病毒性肠胃炎的原因。