One-step cascade detection of glucose at neutral pH based on oxidase-integrated copper(II) metal–organic framework composites,New Journal of Chemistry

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One-step cascade detection of glucose at neutral pH based on oxidase-integrated copper(II) metal–organic framework composites
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-06-30 , DOI: 10.1039/d0nj02550j
Hui Huang _{1,

2,

3,

4} , Wenjing Zhang _{1,

2,

3,

4} , Lulu Lei _{1,

2,

3,

4} , Juan Bai _{1,

2,

3,

4} , Jiao Li _{1,

2,

3,

4} , Donghui Song _{1,

2,

3,

4} , Jingqi Zhao _{1,

2,

3,

4} , Jiali Li _{1,

2,

3,

4} , Yongxin Li _{2,

4,

5,

6}

Affiliation

An integrated system was fabricated from a copper(II) metal–organic framework (Cu-MOF) and glucose oxidase (GOx) for one-step cascade determination of glucose at neutral pH (pH = 7.0). Herein, the Cu-MOF performs dual functions of loading GOx and mimicking peroxidase. The GOx@MOF integrated composites were synthesized under mild conditions. In the integrated system, glucose was oxidized to gluconic acid and hydrogen peroxide by GOx in the presence of oxygen. In the presence of the MOF catalyst, hydrogen peroxide, acting as an intermediate product, was immediately converted to ˙OH, which oxidized 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and UV-vis absorbance at 420 nm was observed. The glucose concentration is positively and linearly correlated with absorbance in the range 8–140 μM with a detection limit of 2.67 μM. Our strategy possesses the characteristics of excellent accuracy, easy operation and biological application. It has been successfully applied to the detection of glucose in human serum, and provides a more convenient and sensitive operation platform for biological analysis.

中文翻译：

基于氧化酶整合的铜（II）金属-有机骨架复合材料的中性pH值一步法级联检测葡萄糖

由铜制成的集成系统（II金属-有机骨架（Cu-MOF）和葡萄糖氧化酶（GOx）用于在中性pH（pH = 7.0）下一步一步测定葡萄糖。在此，Cu-MOF执行装载GOx和模仿过氧化物酶的双重功能。GOx @ MOF集成复合材料是在温和条件下合成的。在集成系统中，葡萄糖在氧气存在下被GOx氧化为葡萄糖酸和过氧化氢。在MOF催化剂的存在下，作为中间产物的过氧化氢立即转化为˙OH，将2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和UV氧化在420nm处观察到可见吸光度。葡萄糖浓度与吸光度在8–140μM范围内呈线性正相关，检出限为2.67μM。我们的策略具有准确性高，易于操作和生物学应用的特点。它已成功应用于人体血清中葡萄糖的检测，为生物学分析提供了更加方便，灵敏的操作平台。

更新日期：2020-07-27

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