Nano-sized extracellular vesicles have become an interesting target for fluorescent labelling due to their complex roles within cellular processes. Extracellular vesicles are released by most cells, including bacteria and mammalian and have demonstrated clear involvement within cellular, as well as patho-physiological processes. They contain information about the cell they originate from and communicate between cells making them promising disease biomarkers and vehicles for drug delivery. Many strategies have been used to fluorescently label extracellular vesicles to track or identify unknown cellular processes. Various targetable proteins are embedded in their membrane enabling them to be fluorescently labelled for detection and identification purposes. The fluorescent probes used for biosensing and bioimaging include organic dyes, fluorescent proteins, metal nanoparticles and quantum dots. Commonly used organic dyes and fluorescent proteins have inherent issues with stability ...

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生物医学应用的细胞外囊泡量子点荧光标记的未来

由于纳米级细胞外囊泡在细胞过程中的复杂作用，它们已成为荧光标记的有趣目标。细胞外囊泡被包括细菌和哺乳动物在内的大多数细胞释放，并已清楚地参与细胞以及病理生理过程。它们包含有关其来源细胞的信息，并在细胞之间进行通讯，使它们成为有希望的疾病生物标志物和药物输送载体。已经使用许多策略来荧光标记细胞外囊泡以追踪或鉴定未知的细胞过程。各种可靶向的蛋白质被嵌入其膜中，从而可以对其进行荧光标记，以进行检测和鉴定。用于生物传感和生物成像的荧光探针包括有机染料，荧光蛋白，金属纳米颗粒和量子点。常用的有机染料和荧光蛋白具有稳定性方面的固有问题。