当前位置: X-MOL 学术J Anal Sci Technol › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A portable standalone wireless electric cell-substrate impedance sensing (ECIS) system for assessing dynamic behavior of mammalian cells
Journal of Analytical Science and Technology ( IF 2.5 ) Pub Date : 2020-06-30 , DOI: 10.1186/s40543-020-00223-9
Uvanesh Kasiviswanathan , Suruchi Poddar , Chandan Kumar , Satyabrata Jit , Sanjeev Kumar Mahto , Neeraj Sharma

In this study, we have developed a wireless, portable, standalone, and simple electric cell-substrate impedance sensing (ECIS) system to analyze in-depth functional aspects of cellular functions on the surface of a co-planar metal electrode coated on conventional glass substrate using a low-cost circuitry and correlated it with an equivalent electrical circuit (EEC) model. Low-cost circuitry was used for studying the dynamic behavior of the mouse myoblast cells (C2C12) in a culture chamber. Further, the developed ECIS system was connected with 8-bit Arduino UNO microcontroller board for establishing a compact sized measuring unit, which can be placed inside a CO 2 incubator to provide proper environmental condition for the biological cells during the entire measuring time. Integrating ZigBee RF module with the 8-bit Arduino UNO microcontroller board provides a wireless communication network. Theoretical calculation of the lumped-elemental electrical parameters associated with cell-electrolyte interface and metal-electrolyte interface was calculated. The calculation was performed by fitting the experimental impedance data to EEC model using least mean square method to determine the dynamic and vital functions of the mammalian cells such as proliferation (in real-time) with a change in intrinsic electrical parameters associated at any particular time point. Impedance measurements and the lumped-elemental electrical parameter were correlated with the respective microscopic images. The developed ECIS system was found to enable measuring of the extent of cellular proliferation over time. The compactness of the developed ECIS system integrated with the ZigBee RF module and the 8-bit Arduino UNO microcontroller board facilitates its utilization even when placed in the CO 2 incubator for a prolonged time.

中文翻译:

一种用于评估哺乳动物细胞动态行为的便携式独立无线电池-基底阻抗传感 (ECIS) 系统

在这项研究中,我们开发了一种无线、便携式、独立和简单的细胞 - 基底阻抗传感 (ECIS) 系统,以分析涂覆在传统玻璃上的共面金属电极表面上细胞功能的深入功能方面基板使用低成本电路,并将其与等效电路 (EEC) 模型相关联。低成本电路用于研究培养室中小鼠成肌细胞 (C2C12) 的动态行为。此外,开发的 ECIS 系统与 8 位 Arduino UNO 微控制器板连接,以建立一个紧凑尺寸的测量单元,该测量单元可以放置在 CO 2 培养箱内,在整个测量时间内为生物细胞提供适当的环境条件。将 ZigBee 射频模块与 8 位 Arduino UNO 微控制器板集成,提供无线通信网络。计算了与电池-电解质界面和金属-电解质界面相关的集总元素电参数的理论计算。通过使用最小均方方法将实验阻抗数据拟合到 EEC 模型来进行计算,以确定哺乳动物细胞的动态和重要功能,例如增殖(实时)与任何特定时间相关的固有电参数的变化观点。阻抗测量和集总元素电参数与相应的显微图像相关。发现开发的 ECIS 系统能够随时间测量细胞增殖的程度。
更新日期:2020-06-30
down
wechat
bug