The need for timely establishment of a complete diagnostic protocol of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is demanded worldwide. We selected 15 positive novel coronavirus disease 19 (COVID-19) patients with mild or no symptom. Initially, fecal samples were negative in the 67% (10/15) of the cases, while 33% (5/10) of the cases were positive. After serial virus RNA testing, 73% (11/15) of the cases resulted positive to fecal specimens. In particular, 15 days after the first positive respiratory specimens test, 6 fecal specimens became positive for SARS-CoV-2 RNA, while 13 respiratory test returned negative result. In conclusion, qRT-PCR assays of fecal specimens, is an important step to control infection, suggesting that samples remained positive for SARS-CoV-2 RNA longer time then respiratory tract samples. Our results enhance the recent knowledge on this emerging infectious disease and offer suggestions for a more complete diagnostic strategy.

中文翻译：

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粪便样品中SARS-CoV-2病毒RNA的评估。

全世界都需要及时建立完整的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）诊断方案。我们选择了15例轻度或无症状的阳性新型冠状病毒病19（COVID-19）患者。最初，粪便样本在67％（10/15）的病例中为阴性，而33％（5/10）的病例为阳性。经过连续病毒RNA检测后，有73％（11/15）的病例对粪便标本呈阳性。特别是在第一次阳性呼吸道标本测试后15天，有6份粪便标本SARS-CoV-2 RNA阳性，而13份呼吸道测试返回阴性结果。总之，粪便标本的qRT-PCR分析是控制感染的重要步骤，这表明与呼吸道标本相比，标本对SARS-CoV-2 RNA的阳性时间更长。