当前位置：
X-MOL 学术
›
Int. J. Nanomed.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Stimulus Responsive Ocular Gentamycin-Ferrying Chitosan Nanoparticles Hydrogel: Formulation Optimization, Ocular Safety and Antibacterial Assessment.
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-06-30 , DOI: 10.2147/ijn.s254763 Nabil K Alruwaili 1 , Ameeduzzafar Zafar 1 , Syed Sarim Imam 2 , Khalid Saad Alharbi 3 , Nasser Hadal Alotaibi 4 , Sultan Alshehri 2, 5 , Nabil A Alhakamy 6 , Abdulaziz I Alzarea 1 , Muhammad Afzal 3 , Mohammed Elmowafy 1, 7
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-06-30 , DOI: 10.2147/ijn.s254763 Nabil K Alruwaili 1 , Ameeduzzafar Zafar 1 , Syed Sarim Imam 2 , Khalid Saad Alharbi 3 , Nasser Hadal Alotaibi 4 , Sultan Alshehri 2, 5 , Nabil A Alhakamy 6 , Abdulaziz I Alzarea 1 , Muhammad Afzal 3 , Mohammed Elmowafy 1, 7
Affiliation
Purpose: The present study was designed to study the gentamycin (GTM)-loaded stimulus-responsive chitosan nanoparticles to treat bacterial conjunctivitis.
Methods: GTM-loaded chitosan nanoparticles (GTM-CHNPs) were prepared by ionotropic gelation method and further optimized by 3-factor and 3-level Box–Behnken design. Chitosan (A), sodium tripolyphosphate (B), and stirring speed (C) were selected as independent variables. Their effects were observed on particle size (PS as Y1), entrapment efficiency (EE as Y2), and loading capacity (LC as Y3).
Results: The optimized formulation showed the particle size, entrapment efficiency, and loading capacity of 135.2± 3.24 nm, 60.18± 1.65%, and 34.19± 1.17%, respectively. The optimized gentamycin-loaded chitosan nanoparticle (GTM-CHNPopt) was further converted to the stimulus-responsive sol-gel system (using pH-sensitive carbopol 974P). GTM-CHNPopt sol-gel (NSG5) exhibited good gelling strength and sustained release (58.99± 1.28% in 12h). The corneal hydration and histopathology of excised goat cornea revealed safe to the cornea. It also exhibited significant (p< 0.05) higher ZOI than the marketed eye drop.
Conclusion: The finding suggests that GTM-CHNP-based sol-gel is suitable for ocular delivery to enhance the corneal contact time and improved patient compliance.
Keywords: chitosan, nanoparticles, gentamycin, histopathology, antimicrobial assessment, HET CAM test
中文翻译：
刺激响应性眼部庆大霉素输送壳聚糖纳米颗粒水凝胶：配方优化、眼部安全性和抗菌评估。
目的：本研究旨在研究负载庆大霉素 (GTM) 的刺激反应性壳聚糖纳米颗粒治疗细菌性结膜炎的效果。
方法：采用离子型凝胶法制备载有 GTM 的壳聚糖纳米颗粒 (GTM-CHNPs)，并通过 3 因子和 3 水平 Box-Behnken 设计进一步优化。选择壳聚糖 (A)、三聚磷酸钠 (B) 和搅拌速度 (C) 作为自变量。观察了它们对粒径（PS 作为 Y1）、包封效率（EE 作为 Y2）和负载能力（LC 作为 Y3）的影响。
结果：优化后的制剂粒径、包封率和载量分别为 135.2± 3.24 nm、60.18± 1.65% 和 34.19± 1.17%。优化后的载有庆大霉素的壳聚糖纳米颗粒 (GTM-CHNPopt) 进一步转化为刺激响应性溶胶-凝胶系统（使用 pH 敏感的卡波姆 974P）。GTM-CHNPopt 溶胶-凝胶 (NSG5) 表现出良好的凝胶强度和持续释放（12 小时内 58.99± 1.28%）。切除的山羊角膜的角膜水合作用和组织病理学显示对角膜是安全的。它还显示出比市售滴眼液显着 (p < 0.05) 更高的 ZOI。
结论：该发现表明基于 GTM-CHNP 的溶胶-凝胶适用于眼部给药，以增加角膜接触时间并提高患者依从性。
关键词：壳聚糖，纳米颗粒，庆大霉素，组织病理学，抗菌评估，HET CAM 测试
更新日期：2020-06-30
Methods: GTM-loaded chitosan nanoparticles (GTM-CHNPs) were prepared by ionotropic gelation method and further optimized by 3-factor and 3-level Box–Behnken design. Chitosan (A), sodium tripolyphosphate (B), and stirring speed (C) were selected as independent variables. Their effects were observed on particle size (PS as Y1), entrapment efficiency (EE as Y2), and loading capacity (LC as Y3).
Results: The optimized formulation showed the particle size, entrapment efficiency, and loading capacity of 135.2± 3.24 nm, 60.18± 1.65%, and 34.19± 1.17%, respectively. The optimized gentamycin-loaded chitosan nanoparticle (GTM-CHNPopt) was further converted to the stimulus-responsive sol-gel system (using pH-sensitive carbopol 974P). GTM-CHNPopt sol-gel (NSG5) exhibited good gelling strength and sustained release (58.99± 1.28% in 12h). The corneal hydration and histopathology of excised goat cornea revealed safe to the cornea. It also exhibited significant (p< 0.05) higher ZOI than the marketed eye drop.
Conclusion: The finding suggests that GTM-CHNP-based sol-gel is suitable for ocular delivery to enhance the corneal contact time and improved patient compliance.
Keywords: chitosan, nanoparticles, gentamycin, histopathology, antimicrobial assessment, HET CAM test
中文翻译：
刺激响应性眼部庆大霉素输送壳聚糖纳米颗粒水凝胶：配方优化、眼部安全性和抗菌评估。
目的：本研究旨在研究负载庆大霉素 (GTM) 的刺激反应性壳聚糖纳米颗粒治疗细菌性结膜炎的效果。
方法：采用离子型凝胶法制备载有 GTM 的壳聚糖纳米颗粒 (GTM-CHNPs)，并通过 3 因子和 3 水平 Box-Behnken 设计进一步优化。选择壳聚糖 (A)、三聚磷酸钠 (B) 和搅拌速度 (C) 作为自变量。观察了它们对粒径（PS 作为 Y1）、包封效率（EE 作为 Y2）和负载能力（LC 作为 Y3）的影响。
结果：优化后的制剂粒径、包封率和载量分别为 135.2± 3.24 nm、60.18± 1.65% 和 34.19± 1.17%。优化后的载有庆大霉素的壳聚糖纳米颗粒 (GTM-CHNPopt) 进一步转化为刺激响应性溶胶-凝胶系统（使用 pH 敏感的卡波姆 974P）。GTM-CHNPopt 溶胶-凝胶 (NSG5) 表现出良好的凝胶强度和持续释放（12 小时内 58.99± 1.28%）。切除的山羊角膜的角膜水合作用和组织病理学显示对角膜是安全的。它还显示出比市售滴眼液显着 (p < 0.05) 更高的 ZOI。
结论：该发现表明基于 GTM-CHNP 的溶胶-凝胶适用于眼部给药，以增加角膜接触时间并提高患者依从性。
关键词：壳聚糖，纳米颗粒，庆大霉素，组织病理学，抗菌评估，HET CAM 测试
京公网安备 11010802027423号