Purpose: The purpose of this study was to investigate the effect of different commercially used dental materials (RelyX Luting Plus and Dyract Extra) mixed with either a metallic ionic solution or a colloidal suspension of metallic nanoparticles. Both the solution and the suspension contained a mixture of silver, copper, and lithium ions.
Methods: The metal/ion-incorporated dental materials were prepared into disk-shaped samples and tested against the growth of Staphylococcus aureus. The susceptibility of bacteria against the antibacterial dental disks was tested using two methods: counting the colony-forming units per milliliter and disk diffusion (Kirby–Bauer). The incorporated materials (Dyract and Rely cement) were tested for ion release using flame atomic absorption spectroscopy.
Results: Assessment showed efficient antibacterial activity of metal ion–incorporated Rely luting cement, exhibited by the formation of inhibition zones larger than those formed by the standard antibiotic, as well as a reduction in bacterial number of sevenfold after incubation for 24 hours. Dyract material incorporated with nanoparticles showed no significant clear zones and had no inhibiting effect on bacterial colony numbers after incubation for 24 hours. The release of silver, copper, and lithium metal ions depended on the type of both dental material and the incorporated nanoagents. The metal ion–incorporated Rely Plus cement released the highest levels of metal ions, which was attributed to its antibacterial efficiency.
Conclusion: Rely Plus cement incorporated with the nanoparticle suspension demonstrated high antibacterial potency, due to the release of the highest concentrations of silver, copper, and lithium metal ions. This work is the first direct comparative study of dental materials with different forms of nanomixtures (metallic nanoparticles and soluble metallic ions) and their antibacterial effects after incubation with bacterial culture for 24 hours.

Keywords: RelyX Luting Plus, Dyract Extra, metallic ionic solution, suspension of metallic nanoparticles, antibacterial activity


中文翻译：

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与纳米金属结合的牙科材料及其对金黄色葡萄球菌细菌生长的影响。

目的：本研究的目的是研究不同商业使用的牙科材料（RelyX Luting Plus 和 Dyract Extra）与金属离子溶液或金属纳米粒子的胶体悬浮液混合的效果。溶液和悬浮液都含有银、铜和锂离子的混合物。
方法：将金属/离子结合的牙科材料制备成圆盘状样品，并测试金黄色葡萄球菌的生长。. 使用两种方法测试细菌对抗菌牙盘的敏感性：计算每毫升的菌落形成单位和牙盘扩散（Kirby-Bauer）。使用火焰原子吸收光谱测试掺入的材料（Dyract 和 Rely 水泥）的离子释放。
结果：评估表明，掺入金属离子的 Rely luting 水泥具有有效的抗菌活性，表现为形成的抑菌圈大于标准抗生素形成的抑菌圈，并且在孵育 24 小时后细菌数量减少了七倍。掺入纳米颗粒的Dyract材料没有显示出明显的透明区域，并且在孵育24小时后对细菌菌落数量没有抑制作用。银、铜和锂金属离子的释放取决于牙科材料和掺入的纳米剂的类型。掺入金属离子的 Rely Plus 水泥释放出最高水平的金属离子，这归因于其抗菌效率。
结论：由于释放最高浓度的银、铜和锂金属离子，与纳米颗粒悬浮液结合的 Rely Plus 水泥表现出很高的抗菌效力。这项工作是第一次对牙科材料与不同形式的纳米混合物（金属纳米颗粒和可溶性金属离子）及其与细菌培养培养 24 小时后的抗菌效果进行直接比较研究。

关键词： RelyX Luting Plus，Dyract Extra，金属离子溶液，金属纳米粒子悬浮液，抗菌活性