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Europium chelate-labeled lateral flow assay for rapid and multiple detection of β-lactam antibiotics by the penicillin-binding protein.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-30 , DOI: 10.1039/d0ay01140a Xiaogang Li 1 , Zhifeng Pan , Manyu Li , Xinmiao Jia , Suhan Zhang , Hang Lin , Juntao Liu , Liangkun Ma
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-30 , DOI: 10.1039/d0ay01140a Xiaogang Li 1 , Zhifeng Pan , Manyu Li , Xinmiao Jia , Suhan Zhang , Hang Lin , Juntao Liu , Liangkun Ma
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For the multiple and rapid detection of β-lactams, the broadly specific penicillin-binding protein (PBP) that recognizes the β-lactam structure was prepared. A chromatographic strip based on europium chelate-loaded fluorescent microspheres was assembled with a goat anti-mouse antibody (C line) and penicillin coating (T line). The penicillin coating competes with free β-lactam antibiotics to bind PBP-labeled fluorescent microspheres. The strip can theoretically detect all kinds of β-lactams, including amoxicillin, ampicillin, oxacillin, ceftazidime, lenampicillin, cefoperazone, sultamicillin, cefotaxime, ceftriaxone, sulbenicillin, piperacillin, cephalothin, flucloxacillin, and mezlocillin, in samples within 10 min. The europium chelate-labeled lateral flow assay does not cross-react with other antibiotics, including chloramphenicol, tetracycline, sulfamethazine, enrofloxacin, gentamicin or lincomycin. In short, we developed a very useful method for preliminary screening of β-lactams.
中文翻译：
che螯合物标记的侧向流动分析法，可通过青霉素结合蛋白快速，多次检测β-内酰胺类抗生素。
为了多重且快速地检测β-内酰胺，制备了识别β-内酰胺结构的广泛特异性的青霉素结合蛋白（PBP）。将基于负载-螯合物的荧光微球的色谱条与山羊抗小鼠抗体（C线）和青霉素涂层（T线）组装在一起。青霉素涂层与游离的β-内酰胺类抗生素竞争，以结合PBP标记的荧光微球。从理论上讲，该试纸条可以检测各种β-内酰胺类药物，包括阿莫西林，氨苄青霉素，奥沙西林，头孢他啶，来氨苄青霉素，头孢哌酮，苏木霉素，头孢噻肟，头孢曲松酮，舒苯西林，哌拉西林，头孢菌素，美洛西林，氟氯西林样品。che螯合物标记的侧向流动测定法不会与其他抗生素（包括氯霉素，四环素，磺胺二甲嘧啶，恩诺沙星，庆大霉素或林可霉素。简而言之，我们开发了一种非常有用的初步筛选β-内酰胺的方法。
更新日期：2020-07-23
中文翻译：
che螯合物标记的侧向流动分析法，可通过青霉素结合蛋白快速，多次检测β-内酰胺类抗生素。
为了多重且快速地检测β-内酰胺，制备了识别β-内酰胺结构的广泛特异性的青霉素结合蛋白（PBP）。将基于负载-螯合物的荧光微球的色谱条与山羊抗小鼠抗体（C线）和青霉素涂层（T线）组装在一起。青霉素涂层与游离的β-内酰胺类抗生素竞争，以结合PBP标记的荧光微球。从理论上讲，该试纸条可以检测各种β-内酰胺类药物，包括阿莫西林，氨苄青霉素，奥沙西林，头孢他啶，来氨苄青霉素，头孢哌酮，苏木霉素，头孢噻肟，头孢曲松酮，舒苯西林，哌拉西林，头孢菌素，美洛西林，氟氯西林样品。che螯合物标记的侧向流动测定法不会与其他抗生素（包括氯霉素，四环素，磺胺二甲嘧啶，恩诺沙星，庆大霉素或林可霉素。简而言之，我们开发了一种非常有用的初步筛选β-内酰胺的方法。
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