The objective of the study was to produce three-dimensional and porous nanofiber reinforced hydrogel scaffolds that can mimic the hydrated composite structure of the cartilage extracellular matrix. In this regard, wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber reinforced carboxymethyl chitosan-silk fibroin (PNFs/CMCht-SF) hydrogel composite scaffolds that were chemically cross-linked by poly(ethylene glycol) diglycidyl ether (PEGDE) were produced. To the best of our knowledge, this is the first study in cartilage regeneration where a three dimensional porous spongy composite scaffold was obtained by the dispersion of wet-electrospun nanofibers within a polymer matrix. All of the produced hydrogel composite scaffolds had an interconnected microporous structure with well-integrated PHBV nanofibers on the pore walls. The scaffold comprising an equal amount of PEGDE and polymer (PNFs/CMCht-SF1:PEGDE1) demonstrated comparable water content (91.4 ± 0.7%), tan δ (0.183 at 1 Hz) and compressive strength (457 ± 85 kPa) values to that of articular cartilage. Besides, based on the histological analysis, this hydrogel composite scaffold supported the chondrogenic differentiation of bone marrow mesenchymal stem cells. Consequently, this hydrogel composite scaffold presented a great promise for cartilage tissue regeneration.

该研究的目的是生产可以模拟软骨细胞外基质的水合复合结构的三维多孔纳米纤维增强水凝胶支架。在这方面，湿电纺聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）纳米纤维增强羧甲基壳聚糖-丝素（PNFs/CMCht-SF）水凝胶复合支架，由聚（乙二醇）化学交联。 ) 生成二缩水甘油醚 (PEGDE)。据我们所知，这是软骨再生的第一项研究，其中通过将湿电纺纳米纤维分散在聚合物基质中获得三维多孔海绵复合支架。所有生产的水凝胶复合支架都具有相互连接的微孔结构，孔壁上有整合良好的 PHBV 纳米纤维。包含等量 PEGDE 和聚合物 (PNFs/CMCht-SF1:PEGDE1) 的支架显示出与此相当的水含量 (91.4 ± 0.7%)、tan δ (0.183 at 1 Hz) 和抗压强度 (457 ± 85 kPa) 值关节软骨。此外，基于组织学分析，这种水凝胶复合支架支持骨髓间充质干细胞的软骨分化。因此，这种水凝胶复合支架为软骨组织再生提供了广阔的前景。这种水凝胶复合支架支持骨髓间充质干细胞的软骨分化。因此，这种水凝胶复合支架为软骨组织再生提供了广阔的前景。这种水凝胶复合支架支持骨髓间充质干细胞的软骨分化。因此，这种水凝胶复合支架为软骨组织再生提供了广阔的前景。