The creation of in vitro functional hepatic tissue simulating micro environmental niche of the native liver is a keen area of research due to its demand in bioartificial liver. However, it is still unclear how to maintain benign cell function while achieving the sufficient cell quantity. In this work, we aim to prepare a novel scaffold for the culture of HepG2 cells, a liver cell line, by modifying polyvinyl alcohol (PVA) scaffold with collagen (COL). PVA is a kind of synthetic biostable polymer with high hydrophilicity in the human body, has been widely used in the biomedical field. However, the use of PVA is limited in cell cultures due to lack of biologically active functional groups. In this study, amino silane (KH-550), glutaraldehyde and native type I collagen were used to modify three-dimensional PVA scaffold to establish a suitable composite scaffold for hepatocyte culture. Three types of composite scaffolds were prepared for different collagen content, named as PVA/COL (0.2%), PVA/COL (0.5%) and PVA/COL (0.8%), respectively. The composite scaffolds were characterized by SEM, XPS, FTIR, MS, porosity estimation and water contact angle measurement. The PVA/COL (0.8%) scaffolds had the highest collagen content of 12.13%. The composite scaffold showed high porosity with interconnected pores. Furthermore, the biocompatibility between HepG2 cells and scaffolds was evaluated by the ability of cell proliferation, albumin secretion, as well as urea synthesis. The coating of collagen on PVA scaffolds promoted hydrophilicity and HepG2 cell adhesion. Additionally, enhanced cell proliferation, increased albumin secretion and urea synthesis were observed in HepG2 cells growing on collagen-coated three-dimensional PVA scaffolds.

由于其对生物人工肝的需求，模拟天然肝脏微环境生态位的体外功能性肝组织的创建是一个敏锐的研究领域。然而，如何在达到足够的细胞数量的同时维持良性细胞功能仍不清楚。在这项工作中，我们的目标是通过用胶原蛋白 (COL) 修饰聚乙烯醇 (PVA) 支架来制备用于培养肝细胞系 HepG2 细胞的新型支架。PVA是一种在人体内具有高亲水性的合成生物稳定聚合物，已广泛应用于生物医学领域。然而，由于缺乏生物活性官能团，PVA 在细胞培养中的使用受到限制。在本研究中，氨基硅烷 (KH-550)、使用戊二醛和天然I型胶原对三维PVA支架进行修饰，建立适合肝细胞培养的复合支架。针对不同的胶原含量制备了三种类型的复合支架，分别命名为 PVA/COL（0.2%）、PVA/COL（0.5%）和 PVA/COL（0.8%）。通过SEM、XPS、FTIR、MS、孔隙率估计和水接触角测量对复合支架进行表征。PVA/COL (0.8%) 支架的胶原蛋白含量最高，为 12.13%。复合支架显示出高孔隙率和相互连接的孔隙。此外，HepG2 细胞与支架之间的生物相容性通过细胞增殖、白蛋白分泌以及尿素合成的能力进行评估。PVA 支架上的胶原涂层促进了亲水性和 HepG2 细胞粘附。此外，