Background:

miR-377 is closely related to myocardial regeneration. miR-377-adjusted mesenchymal stem cells abducted ischemic cardiac angiogenesis. Nevertheless, there were rarely reports about the impact of miR-377 on myocardial ischemia injury. The purpose of this work is that whether miR-377 can protect against myocardial injury caused by hypoxia/reoxygenation (H/R).

Methods:

Gene expression omnibus database (http://www.ncbi.nlm.nih.gov/geo/; no. GSE53211) was utilized to study the differential expression of miR-377 in patients with an acute ST-segment elevation myocardial infarction and healthy controls. The luciferase activity was determined utilizing the dual-luciferase reporter system. Quantitative real-time polymerase chain reaction and Western blotting were used to measure the messenger RNA and protein level.

Results:

Low expression of miR-377 and high expression of leukocyte immunoglobulin-like receptor B2 (LILRB2) were identified in patients with myocardial infarction from analyzing the Gene Expression Omnibus data set. Besides, miR-377 expression was downregulated in cardiomyocyte exposed to H/R. Additionally, overexpression of miR-377 could visibly improve cardiomyocyte injury by regulating cell activity and apoptosis.

Conclusions:

In short, our findings suggested that miR-377/LILRB2 might regard as a hopeful therapeutic target for myocardial ischemic.

中文翻译：

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MicroRNA-377 通过下调 LILRB2 表达减轻缺氧/复氧引起的心肌损伤。

背景：

miR-377与心肌再生密切相关。miR-377 调整的间充质干细胞外展缺血性心脏血管生成。然而，很少有关于 miR-377 对心肌缺血损伤影响的报道。这项工作的目的是研究 miR-377 是否可以防止因缺氧/复氧 (H/R) 引起的心肌损伤。

方法：

利用基因表达综合数据库（http://www.ncbi.nlm.nih.gov/geo/；编号 GSE53211）研究 miR-377 在急性 ST 段抬高心肌梗死患者和健康人中的差异表达控制。使用双荧光素酶报告系统测定荧光素酶活性。定量实时聚合酶链反应和蛋白质印迹用于测量信使 RNA 和蛋白质水平。

结果：

通过分析基因表达综合数据集，在心肌梗塞患者中鉴定出 miR-377 的低表达和白细胞免疫球蛋白样受体 B2 (LILRB2) 的高表达。此外，miR-377 表达在暴露于 H/R 的心肌细胞中下调。此外，过表达 miR-377 可通过调节细胞活性和凋亡明显改善心肌细胞损伤。

结论：

简而言之，我们的研究结果表明 miR-377/LILRB2 可能被视为心肌缺血的有希望的治疗靶点。