Metabolism of SCCPs and MCCPs in Suspension Rice Cells Based on Paired Mass Distance (PMD) Analysis.,Environmental Science & Technology

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Metabolism of SCCPs and MCCPs in Suspension Rice Cells Based on Paired Mass Distance (PMD) Analysis.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-06-29 , DOI: 10.1021/acs.est.0c01830
Weifang Chen _{1,

2} , Miao Yu ₃ , Qing Zhang _{1,

2} , Xingwang Hou _{1,

2,

4} , Wenqian Kong _{1,

2} , Linfeng Wei _{1,

2} , Xiaowei Mao ₅ , Jiyan Liu _{1,

2,

4} , Jerald L Schnoor ₆ , Guibin Jiang _{1,

2,

4}

Affiliation

Short-chain and medium-chain chlorinated paraffins (SCCPs and MCCPs) are mixtures of complex chemical compounds with intensive usage. They are frequently detected in various environmental samples. However, the interaction between CPs and plants, especially the biotransformation behaviors of CPs within plants, is poorly understood. In this study, 1,2,5,6,9,10-hexachlorodecane (CP-4, a typical standard of individual SCCP congeners) and 52%-MCCP (a commercial mixture standard of MCCPs with 52% chlorine content by mass) were selected as representative chemicals to explore the metabolic behaviors of SCCPs and MCCPs using suspension rice cell culture exposure systems. Both 79.53% and 40.70% of CP-4 and 52%-MCCP were metabolized by suspension rice cells, respectively. A complementary suspected screening strategy based on the pair mass distances (PMD) analysis algorithm was used to study the metabolism of CPs mediated by the plant cells. Forty and 25 metabolic products for CP-4 and 52%-MCCP, respectively, were identified, including (multi-) hydroxylation, dechlorination, −HCl– elimination metabolites, (hydroxylation-) sulfation, and glycosylation conjugates. Here, we propose a comprehensive metabolic molecular network and provide insight on degradation pathways of SCCPs and MCCPs in plants for the first time, aiding in further understanding of the transformation behaviors of CPs.

中文翻译：

基于配对质量距离 (PMD) 分析的悬浮稻细胞中 SCCPs 和 MCCPs 的代谢。

短链和中链氯化石蜡（SCCPs 和 MCCPs）是复杂化合物的混合物，使用频繁。它们经常在各种环境样品中检测到。然而，CPs与植物之间的相互作用，尤其是CPs在植物内的生物转化行为，却知之甚少。在本研究中，1,2,5,6,9,10-六氯癸烷（CP-4，单个 SCCP 同类物的典型标准）和 52%-MCCP（氯含量为 52% 的 MCCP 的商业混合标准，按质量计）被选为代表性化学品，使用悬浮水稻细胞培养暴露系统探索 SCCPs 和 MCCPs 的代谢行为。分别有 79.53% 和 40.70% 的 CP-4 和 52%-MCCP 被悬浮稻细胞代谢。基于对质量距离 (PMD) 分析算法的互补疑似筛选策略用于研究由植物细胞介导的 CP 代谢。分别鉴定了 CP-4 和 52%-MCCP 的 40 和 25 种代谢产物，包括（多）羟基化、脱氯、-HCl-消除代谢物、（羟基化-）硫酸化和糖基化缀合物。在这里，我们提出了一个全面的代谢分子网络，并首次深入了解植物中 SCCPs 和 MCCPs 的降解途径，有助于进一步了解 CPs 的转化行为。-HCl-消除代谢物、（羟基化-）硫酸化和糖基化结合物。在这里，我们提出了一个全面的代谢分子网络，并首次深入了解植物中 SCCPs 和 MCCPs 的降解途径，有助于进一步了解 CPs 的转化行为。-HCl-消除代谢物、（羟基化-）硫酸化和糖基化结合物。在这里，我们提出了一个全面的代谢分子网络，并首次深入了解植物中 SCCPs 和 MCCPs 的降解途径，有助于进一步了解 CPs 的转化行为。

更新日期：2020-08-18

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