Optical microscopy is an indispensable tool in biomedical sciences, but its reach in deep tissues is limited due to aberrations and scattering. This problem can be overcome by wavefront-shaping techniques, albeit at limited fields of view (FOVs). Inspired by astronomical imaging, conjugate wavefront shaping can lead to an increased field of view in microscopy, but this correction is limited to a set depth and cannot be dynamically adapted. Here, we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing (F-SHARP). We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes. We demonstrate the power of the method by imaging with high resolution over extended FOV (>80 µm) deeper than 400 μm inside a mouse brain through a thinned skull.

光学显微镜是生物医学科学中不可或缺的工具，但由于像差和散射，其在深层组织中的应用受到限制。这个问题可以通过波前整形技术来克服，尽管在有限的视场 (FOV) 下。受天文成像的启发，共轭波前整形可以增加显微镜的视野，但这种校正仅限于设定的深度，不能动态适应。在这里，我们提出了一种基于聚焦扫描全息像差探测 (F-SHARP) 的共轭波前整形方案。我们将它与紧凑的实现相结合，可以很容易地适应各种商业和自制的双光子显微镜。我们通过在扩展 FOV (>