Ammonia-oxidizing bacteria (AOB) and aerobic methane oxidizing-bacteria (MOB) were studied in three extreme soils of the former Lake Texcoco, Mexico, with pH ranging from 8.5 to 10.5 and electrolytic conductivity (EC) from 0.67 to 84.76 dS m⁻¹, and in two arable soils. Soil DNA was extracted with three different methods and total DNA was used as a template to amplify the pmoA and amoA functional genes and subsequently sequenced by pyrosequencing. The amoA gene sequences clustered as uncultured AOB dominated in the Texcoco soils, while Nitrosospira was dominant in the arable soils. Sequences of MOB associated with Nitrosococcus-rel (Type I) dominated (>85%) in the Texcoco soils, but they were more diverse in the arable soils, for example, JR2, JR3, Methylocaldum USC-g (Type I), USC-a (Type II) and gp23 (pxmA). Aerobic methane oxidizing-bacteria and AOB microbial diversity were significantly related to EC and pH (p < 0.05). As such, the lower MOB and AOB microbial diversity in the Texcoco soil compared to the arable soil was determined by its higher EC and pH.

氨氧化细菌（AOB）和好氧甲烷氧化菌（MOB）进行了研究前特斯科科湖，墨西哥三个极端土壤，pH范围从8.5至0.67至84.76德尚中号10.5和电解电导率（EC）^{- 1}，并且在两个耕地中。用三种不同的方法提取土壤DNA，并将总DNA用作模板以扩增pmoA和amoa功能基因，然后通过焦磷酸测序进行测序。所述AMOA基因序列聚类如特斯科科土壤主导未培养的AOB，而Nitrosospira是在可耕地中占主导地位。与硝化球菌相关的MOB序列-rel（I型）在Texcoco土壤中占主导地位（> 85％），但在可耕土壤中它们的多样性更高，例如JR2，JR3，Methylocaldum USC-g（I型），USC-a（II型）和gp23（pxmA）。好氧甲烷氧化细菌和AOB微生物多样性与EC和pH显着相关（p  <0.05）。因此，与可耕土壤相比，特可可土壤中较低的MOB和AOB微生物多样性是由其较高的EC和pH决定的。