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Biodegradation Effect on the Campanian Oil Shale of Egypt
Geomicrobiology Journal ( IF 2.2 ) Pub Date : 2020-06-30
Huda Saleh El-Sheshatwy, Nagla Khalil, Sherif Farouk

In the present article, ten bacterial strains were isolated from two Campanian bulk shale samples from Abu-Tartur mine of the Western Desert and the Quseir area at the El-Nakheil area of Red Sea, Egypt. The characterization of these two samples was analyzed. Two of the bacterial isolated strains showed a higher growth rate on oil shale and these were selected for the biodegradation process. These bacterial isolates (B1 and B2) were identified using molecular identification performed by 16S rRNA gene sequences. The results indicated that two isolated bacteria belong to Bacillus flexus and Enterobacter cloacae. Gas Chromatographic analysis of the remaining oil shale in different culture medium after one week at 30 oC yielded optimum percentage biodegradation at 35.15% for the Abu-Tartur oil shale sample, while for the El- Nakheil oil shale sample was detected at 32.13%. The bacterial strain (B1) consumed more of the iso-paraffins than n-paraffins of saturates fraction in the Abu-Tartur oil shale sample. The complete utilization of some different polyaromatics membered rings and the highest percentage biodegradation of other polyaromatics were determined with two bacterial strains and different oil shale samples separately. From the results seems to be worthy biodegradation of oil shale samples trend.



中文翻译:

对埃及坎帕尼亚油页岩的生物降解作用

在本文中,从埃及西部沙漠和埃及红海El-Nakheil地区的Quseir地区的两个Campanian散页岩样品中分离出10个细菌菌株。分析了这两个样品的表征。其中两个细菌分离株在油页岩上显示出较高的生长速率,并选择了它们进行生物降解。这些细菌分离株(B1和B2)使用16S rRNA基因序列进行分子鉴定来鉴定。结果表明,分离到的细菌为弯曲芽孢杆菌阴沟肠杆菌。30 o一周后在不同培养基中剩余油页岩的气相色谱分析对于Abu-Tartur油页岩样品,C产生的最佳生物降解百分比为35.15%,而El-Nakheil油页岩样品的生物降解率为32.13%。在Abu-Tartur油页岩样品中,细菌菌株(B1)消耗的异链烷烃比饱和馏分的正链烷烃要多。分别用两种细菌菌株和不同的油页岩样品确定了某些不同的多芳族元环的完全利用和其他多芳族化合物最高的生物降解百分比。从结果看似值得生物降解油页岩样品的趋势。

更新日期:2020-06-30
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