ABSTRACT The objectives of this study were to produce dog food containing curcumin replacing synthetic antioxidants, to evaluate its beneficial effects on animal growth and health. Curcumin (100 mg/kg) was added after the extrusion process along with the other micronutrients. The final concentration of curcumin was 32.9 mg/kg. The control feed was composed of the same ingredients without curcumin. After a storage of 6 months, feed composition and pH did not differ; however, the feed with curcumin showed lower protein oxidation, lipid peroxidation and higher total antioxidant capacity. After 2 months of feed production, 12 young Beagle dogs received either curcumin-containing food (n = 6) or the control diet (n = 6). The animals were fed twice a day using individual kennels. Blood samples were taken on d 1, 35 and 42. During the first 30 d of the study, the animals had natural infectious diseases that were controlled with anti-protozoals and antibiotics. Greater numbers of red blood cells were observed in dogs fed with curcumin (d 35 and 45), and there were greater numbers of white blood cells as a consequence of increased neutrophils on d 42. At the end of the experiment, a significant reduction in the number of lymphocytes was observed in dogs that ingested curcumin (d 42), suggesting an anti-inflammatory effect, manifested as a decrease in globulin levels. In the final 15 d of the experiment, the animals were clinical healthy. Higher serum levels of glucose, urea, triglycerides and cholesterol were observed in dogs fed with curcumin. Curcumin increased the activity of several antioxidant enzymes in addition to non-protein thiols and the total antioxidant capacity in the serum, consequently reducing levels of oxygen reactive species. Curcumin supplementation of dogs did not favour growth or weight gain. Neverthless, it was concluded that curcumin improved animal health, with emphasis on the stimulation of the antioxidant system and evidence of an anti-inflammatory effect.

中文翻译：

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使用姜黄素作为抗氧化剂生产狗粮：摄入量对动物生长、健康和饲料保护的影响

摘要 本研究的目的是生产含有姜黄素的狗粮以替代合成抗氧化剂，以评估其对动物生长和健康的有益影响。姜黄素 (100 mg/kg) 在挤压过程后与其他微量营养素一起加入。姜黄素的最终浓度为 32.9 mg/kg。对照饲料由相同成分组成，不含姜黄素。储存 6 个月后，饲料成分和 pH 值没有变化；然而，含有姜黄素的饲料表现出较低的蛋白质氧化、脂质过氧化和较高的总抗氧化能力。经过 2 个月的饲料生产，12 只年轻的比格犬接受了含姜黄素的食物（n = 6）或对照饮食（n = 6）。使用单独的狗舍每天给动物喂食两次。在第 1、35 和 42 天采集血样。在研究的前 30 天，这些动物患有自然传染病，这些疾病用抗原生动物和抗生素加以控制。在喂食姜黄素的狗（第 35 天和第 45 天）中观察到更多数量的红细胞，并且由于第 42 天中性粒细胞增加，白细胞数量更多。在实验结束时，白细胞数量显着减少在摄入姜黄素的狗中观察到淋巴细胞的数量（第 42 天），表明具有抗炎作用，表现为球蛋白水平降低。在实验的最后 15 天，动物临床健康。在喂食姜黄素的狗中观察到较高的葡萄糖、尿素、甘油三酯和胆固醇的血清水平。除了非蛋白质硫醇和血清中的总抗氧化能力外，姜黄素还增加了几种抗氧化酶的活性，从而降低了氧活性物质的水平。狗的姜黄素补充剂不利于生长或体重增加。尽管如此，得出的结论是姜黄素改善了动物的健康，重点是刺激抗氧化系统和抗炎作用的证据。