Abstract In this investigation, we have designed and synthesized a new Ru(II)-arene complex with triarylamine thiosemicarbazone hybrid ligand to explore its cytotoxicity. The ligand and the complex were well characterized by spectroscopic techniques. From the single-crystal X-ray crystallography, the molecular structure of the ligand was confirmed to be monoclinic lattice with C12/C1 space group symmetry. X-ray photoelectron spectroscopy(XPS) studies ensured that ruthenium is in +2 oxidation state. The synthesized Ru(II)-arene complex was thoroughly investigated for the in vitro activity against human breast carcinoma (MCF-7), human colon carcinoma (COLO 205), human neuroblastoma (IMR-32), murine microphage (Raw 264.7) and embryonic kidney (HEK 293) using MTT assay. The interaction of the Ru(II)-arene complex with DNA/protein was also explored by absorption and emission spectral methods. This investigation highlights the role of hybrid ligand and its Ru(II) complex toward high cytotoxicity in vitro. The complex has high cytotoxic effect with IC50 value of 5.18 ± 1.128 µM toward human breast carcinoma cell line. Graphical Abstract

中文翻译：

---

结构不同的域嵌入半夹心芳烃 Ru(II) 复合物：DNA/HSA 结合和细胞毒性研究

摘要 在这项研究中，我们设计并合成了一种新的 Ru(II)-芳烃配合物与三芳胺缩氨基硫脲杂化配体，以探索其细胞毒性。配体和配合物通过光谱技术得到了很好的表征。从单晶X射线晶体学证实配体的分子结构为具有C12/C1空间群对称性的单斜晶格。X 射线光电子能谱 (XPS) 研究确保钌处于 +2 氧化态。对合成的 Ru(II)-芳烃复合物对人乳腺癌 (MCF-7)、人结肠癌 (COLO 205)、人神经母细胞瘤 (IMR-32)、鼠巨噬细胞 (Raw 264.7) 和使用 MTT 测定的胚胎肾 (HEK 293)。还通过吸收和发射光谱方法探索了 Ru(II)-芳烃复合物与 DNA/蛋白质的相互作用。这项研究突出了混合配体及其 Ru(II) 复合物在体外对高细胞毒性的作用。该复合物对人乳腺癌细胞系具有高细胞毒作用，IC50 值为 5.18 ± 1.128 µM。图形概要