Abstract

Prunus amygdalus (PA) is a popular invasive seed utilized in the management of diabetes in Jammu and Kashmir, India. The objective of the current study was to scrutinize the antidiabetic effect of Prunus amygdalus (PA) against Streptozotocin (STZ) induced diabetic rats and explore the possible mechanism of action at cellular and sub-cellular levels. Box Benkan Design (BBD) was performed to determine the effect of PA powder to methanol, extraction time and extraction temperature on DPPH and ABTS free radical scavenging activity of decoction. In-silico study was performed on GLUT1 (5EQG) and dipeptidyl peptidase IV (DPPIV) (2G63) protein. Type II diabetes mellitus was initiated by single intra-peritoneal injection of STZ. The Blood Glucose Level (BGL) and body weight were estimated at regular interval of time. The different biochemical parameters such as hepatic, antioxidant, and lipid parameters were estimated. At end of the study, pancreas was used for histopathological observation. The variation in DPPH antiradical scavenging activity 40.0–90.0% and ABTS antiradical scavenging activity 34–82%, were estimated respectively. STZ induced DM rats showed increased BGL at end of the experimental study. PA treatment significantly (p < 0.001) down-regulated the BGL level. PA significantly (p < 0.001) altered the biochemical, hepatic and antioxidant parameters in a dose-dependent manner. Histopathological examination demonstrated the constructive mass of β-cells in pancreas. Overall, the current study indicates that the PA treatment down-regulated the hyperglycemic, oxidative stress and hyperlipidaemia in diabetic rats, due to inhibition of enzymes or amelioration of oxidative stress.

Communicated by Ramaswamy H. Sarma

摘要

Prunus amygdalus ( PA ) 是一种流行的侵入性种子，用于印度查谟和克什米尔的糖尿病管理。本研究的目的是检查杏仁(PA) 对链脲佐菌素 (STZ) 诱导的糖尿病大鼠的抗糖尿病作用，并探索细胞和亚细胞水平的可能作用机制。Box Benkan Design (BBD) 用于确定 PA 粉末对甲醇、提取时间和提取温度对煎剂的 DPPH 和 ABTS 自由基清除活性的影响。硅片对 GLUT1 (5EQG) 和二肽基肽酶 IV (DPPIV) (2G63) 蛋白进行了研究。II 型糖尿病由单次腹膜内注射 STZ 引发。定期估计血糖水平 (BGL) 和体重。估计了不同的生化参数，例如肝脏、抗氧化剂和脂质参数。在研究结束时，使用胰腺进行组织病理学观察。分别估计 DPPH 抗自由基清除活性的变化 40.0-90.0% 和 ABTS 抗自由基清除活性的 34-82%。STZ 诱导的 DM 大鼠在实验研究结束时显示出增加的 BGL。PA处理显着 ( p  < 0.001) 下调 BGL 水平。PA显着 ( p < 0.001) 以剂量依赖性方式改变生化、肝脏和抗氧化参数。组织病理学检查表明胰腺中存在建设性的 β 细胞团块。总体而言，目前的研究表明，由于酶的抑制或氧化应激的改善，PA治疗下调了糖尿病大鼠的高血糖、氧化应激和高脂血症。

由 Ramaswamy H. Sarma 交流