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Occurrence and distribution of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Morocco
Biocontrol Science and Technology ( IF 1.5 ) Pub Date : 2020-06-30
Youssef Benseddik, Abdelmalek Boutaleb Joutei, Abdelali Blenzar, Said Ezrari, Carlos M. Molina, Nabil Radouane, Fouad Mokrini, Abdessalem Tahiri, Rachid Lahlali, Abdelfettah A. Dababat

A survey of entomopathogenic nematodes (EPN) was conducted in Morocco during 2015 and 2016 in three regions with varying topography, climate and vegetation (Meknès-El Hajeb, Ifrane and Tafilalet). EPN were isolated from 14 of 169 soil samples (8%). Molecular and morphological techniques were used for species determination. Nine samples contained heterorhabditids and five samples contained steinernematids. Eight heterorhabditids were identified as Heterorhabditis bacteriophora Poinar 1976, two steinernematids as Steinernema feltiae (Filipjev, 1934), three as belonging to the S. feltiae-group, and one as an unidentified isolate (Heterorhabditis sp.) which gives dark burgundy colour for G. mellonella cadavers. These EPNs were collected mainly from agricultural soils rather than natural habitats, with potential adaptation to specific geography, climate and soil conditions (texture, pH and organic matter content), including six EPN isolates from heavy soils. These data demonstrate that EPN are present in Moroccan soils but at relatively low frequency, so opportunity exists to augment these populations to gain biological control benefits.



中文翻译:

昆虫病原线虫(Steinernematidae和Heterorhabditidae)的发生和分布

2015年和2016年在摩洛哥对地形,气候和植被各不相同的三个地区(Meknès-ElHajeb,Ifrane和Tafilalet)进行了昆虫致病线虫调查(EPN)。从169个土壤样品中的14个(占8%)中分离出了EPN。分子和形态学技术用于物种确定。九个样品中含有异种生殖,五个样品中含有类神经炎。八个heterorhabditids被确定为异小嗜菌Poinar 1976年,二steinernematids为斯氏线虫(Filipjev,1934年)三成属于S.氏线虫-group,和一个作为一个身份不明的分离(异小SP。),这也是暗酒红色G. mellonella尸体。这些EPNs主要是从农业土壤而不是自然栖息地收集的,可能适应特定的地理,气候和土壤条件(质地,pH和有机质含量),包括从重土壤中分离出的6种EPN。这些数据表明,EPN存在于摩洛哥土壤中,但发生频率相对较低,因此存在扩大这些种群的机会,从而获得生物防治效益。

更新日期:2020-06-30
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